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首页> 外文期刊>Archives of Biochemistry and Biophysics >Molecular network including eIF1AX, RPS7, and 14-3-3 gamma regulates protein translation and cell proliferation in bovine mammary epithelial cells
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Molecular network including eIF1AX, RPS7, and 14-3-3 gamma regulates protein translation and cell proliferation in bovine mammary epithelial cells

机译：包括eIF1AX，RPS7和14-3-3γ在内的分子网络调节牛乳腺上皮细胞中的蛋白质翻译和细胞增殖

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14-3-3 gamma, an isoform of the 14-3-3 protein family, was proved to be a positive regulator of mTOR pathway. Here, we analyzed the function of 14-3-3 gamma in protein synthesis using bovine mammary epithelial cells (BMECs). We found that 14-3-3 gamma interacted with eIF1AX and RPS7 by 14-3-3 gamma coimmunoprecipitation (CoIP) and matrix-assisted laser desorption/ionization-time-of-flight/time-of-flight (MALDI-TOF/TOF) peptide mass fingerprinting analysis. These interactions of 14-3-3 gamma with eIF1AX and RPS7 were further confirmed by colocalization and fluorescence resonance energy transfer (FRET) analysis. We also found that methionine could promote protein synthesis and trigger the protein expression levels of 14-3-3 gamma, eIF1AX and RPS7. Analysis of overexpression and inhibition of 14-3-3 gamma confirmed that it positively affected the protein expression levels of eIF1AX, RPS7, Stat5 and mTOR pathway to promote protein synthesis and cell proliferation in BMECs. We further showed that overexpression of eIF1AX and RPS7 also triggered protein translation and cell proliferation. From these results, we conclude that molecular network including eIF1AX, RPS7, and 14-3-3 gamma regulates protein translation and cell proliferation in BMECs. (C) 2014 Elsevier Inc. All rights reserved.
机译：14-3-3γ是14-3-3蛋白家族的同工型，被证明是mTOR途径的正调节剂。在这里，我们分析了14-3-3γ在使用牛乳腺上皮细胞（BMEC）进行蛋白质合成中的功能。我们发现14-3-3γ通过14-3-3γ免疫共沉淀（CoIP）和基质辅助激光解吸/电离飞行时间/飞行时间（MALDI-TOF / TOF）肽质量指纹分析。共定位和荧光共振能量转移（FRET）分析进一步证实了14-3-3γ与eIF1AX和RPS7的相互作用。我们还发现蛋氨酸可以促进蛋白质合成并触发14-3-3γ，eIF1AX和RPS7的蛋白质表达水平。对14-3-3γ的过表达和抑制作用的分析证实，它积极影响eIF1AX，RPS7，Stat5和mTOR途径的蛋白表达水平，从而促进BMEC中的蛋白合成和细胞增殖。我们进一步表明，eIF1AX和RPS7的过表达也触发了蛋白质翻译和细胞增殖。根据这些结果，我们得出结论，包括eIF1AX，RPS7和14-3-3γ在内的分子网络调节BMEC中的蛋白质翻译和细胞增殖。 （C）2014 Elsevier Inc.保留所有权利。

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