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首页> 外文期刊>Archives of medical research >Expression of discoidin domain receptors (DDR2) in alcoholic liver fibrosis in rats.
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Expression of discoidin domain receptors (DDR2) in alcoholic liver fibrosis in rats.

机译：盘状蛋白结构域受体（DDR2）在大鼠酒精性肝纤维化中的表达。

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BACKGROUND AND AIMS: We undertook this study to evaluate the expression of discoidin domain receptor 2 (DDR2) and its relationship with alcoholic liver fibrosis. METHODS: Liver fibrosis was induced by intragastric administration of alcohol in 30 rats. Pathological changes and ultrastructure of the liver were studied. The expression of DDR2 mRNA and protein was detected by RT-PCR and Western blot, respectively, at weeks 12, 16 and 20 during the alcohol administration. RESULTS: In the control group (con) (n = 10), DDR2 mRNA expression and DDR2 protein were 1.02 +/- 0.13 (con ratio x10(3)) and 0.32 +/- 0.03, respectively. In the study groups there was a progressive increase in DDR2 mRNA expression from weeks 12, 16 and 20 (3.64 +/- 1.69, 8.34 +/- 2.39, 15.73 +/- 4.57 con ratio x10(3), p <0.05). There was also a progressive increase in DDR2 protein from weeks 12-20 (0.48 +/- 0.05, 0.74 +/- 0.06 and 0.99 +/- 0.05, p <0.05). The mean DDR2 mRNA and protein in the three study groups was higher than in the control group (p <0.01). The expressions of DDR2 mRNA and protein were positively correlated with collagen type I, III and IV in liver tissues as well as with the serum biomarkers of liver fibrosis, collagen type III, hyaluronic acid, collagen type IV and laminin (p <0.01). CONCLUSIONS: The expression of DDR2 in this alcohol-induced liver fibrosis rat model is enhanced. The expression of DDR2 is closely associated with collagens in the fibrotic liver tissues.
机译：背景与目的：我们进行了这项研究，以评估盘状蛋白结构域受体2（DDR2）的表达及其与酒精性肝纤维化的关系。方法：30只大鼠灌胃饮酒可引起肝纤维化。研究了肝脏的病理变化和超微结构。酒精给药期间第12、16和20周分别通过RT-PCR和Western blot检测DDR2 mRNA和蛋白的表达。结果：对照组（对照组）（n = 10）中，DDR2 mRNA表达和DDR2蛋白分别为1.02 +/- 0.13（对比率x10（3））和0.32 +/- 0.03。在研究组中，从第12、16和20周开始，DDR2 mRNA表达逐渐增加（3.64 +/- 1.69、8.34 +/- 2.39、15.73 +/- 4.57对比率x10（3），p <0.05）。从第12-20周开始，DDR2蛋白也逐渐增加（0.48 +/- 0.05、0.74 +/- 0.06和0.99 +/- 0.05，p <0.05）。三个研究组的平均DDR2 mRNA和蛋白均高于对照组（p <0.01）。 DDR2 mRNA和蛋白的表达与肝组织中的I，III和IV型胶原蛋白以及肝纤维化，III型胶原蛋白，透明质酸，IV型胶原蛋白和层粘连蛋白的血清生物标志物呈正相关（p <0.01）。结论：DDR2在酒精性肝纤维化大鼠模型中的表达增强。 DDR2的表达与纤维化肝组织中的胶原蛋白密切相关。

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