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首页> 外文期刊>Archives of dermatological research. >Vitamin D increases expression of cathelicidin in cultured sebocytes
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Vitamin D increases expression of cathelicidin in cultured sebocytes

机译：维生素D增加培养的皮脂细胞中cathelicidin的表达

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Antimicrobial peptides (AMPs) have been widely accepted as major contributors to cutaneous innate non-specific immunity, which is the first line of defense against invading pathogens. Altered expression of AMPs has been described in chronic inflammatory disorders of the skin. This study was conducted to clarify the expression of AMPs, including psoriasin, human β-defensin (hBD)-2, hBD-3, and LL-37, in cultured sebocytes and to evaluate the changes in the expression of AMPs in cultured sebocytes after treatment with vitamin D. Primary culture of sebocytes obtained from hair follicles of the scalp was used for this study. Cultured sebocytes were treated with vitamin D (10 -10-10 -6 M). In addition, vitamin D (10 -10, 10 -8 and 10 -6 M) was added to sebocytes treated with vitamin D receptor (VDR) siRNA. Reverse transcription-polymerase chain reaction for psoriasin, hBD-2, hBD-3, and hCAP-18 was performed using cultured sebocytes and sebocytes treated with VDR siRNA. Western blotting for psoriasin, hBD-2, hBD-3, and hCAP-18 was also performed using sebocytes treated with vitamin D (10 -10, 10 -8, and 10 -6 M). Gene and protein expression of hCAP-18 (p0.05) showed an increase in sebocytes treated with vitamin D. Gene expression of hCAP-18 by treatment with vitamin D was blocked in sebocytes treated with VDR siRNA. In conclusion, treatment with vitamin D resulted in increased expression of LL-37 through the vitamin D receptor of cultured sebocytes.
机译：抗菌肽（AMPs）已被广泛接受为皮肤固有非特异性免疫的主要贡献者，这是抵御入侵病原体的第一道防线。在皮肤的慢性炎性疾病中已经描述了AMPs的表达改变。进行这项研究以阐明培养的皮细胞中AMPs的表达，包括牛皮癣菌素，人β-防御素（hBD）-2，hBD-3和LL-37，并评估AMPs在培养后的皮脂细胞中的表达变化。维生素D的治疗。从头皮毛囊获得的皮脂细胞的原代培养用于这项研究。用维生素D（10 -10-10 -6 M）处理培养的皮脂细胞。此外，将维生素D（10 -10、10 -8和10 -6 M）添加到用维生素D受体（VDR）siRNA处理的皮脂细胞中。使用培养的皮脂细胞和经VDR siRNA处理的皮脂细胞对牛皮癣菌素，hBD-2，hBD-3和hCAP-18进行逆转录聚合酶链反应。还使用维生素D（10 -10、10 -8和10 -6 M）处理的皮脂细胞对牛皮癣菌素，hBD-2，hBD-3和hCAP-18进行了蛋白质印迹。 hCAP-18的基因和蛋白质表达（p <0.05）显示维生素D处理的皮脂细胞增加。维生素D处理的hCAP-18的基因表达在VDR siRNA处理的皮脂细胞中被阻断。总之，用维生素D处理可通过培养的皮脂细胞的维生素D受体增加LL-37的表达。

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