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首页> 外文期刊>Archives of dermatological research. >Histamine release-neutralization assay for sera of patients with atopic dermatitis and/or cholinergic urticaria is useful to screen type I hypersensitivity against sweat antigens
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Histamine release-neutralization assay for sera of patients with atopic dermatitis and/or cholinergic urticaria is useful to screen type I hypersensitivity against sweat antigens

机译：组胺释放中和试验用于特应性皮炎和/或胆碱能性荨麻疹患者的血清可用于筛选针对汗液抗原的I型超敏反应

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We previously reported that about 80 % of patients with atopic dermatitis and 60 % with cholinergic urticaria revealed type I allergy against sweat, by means of skin test against autologous sweat and/or histamine-release test for peripheral blood basophils with semi-purified sweat antigen. In this study, we developed an assay for sera to neutralize histamine-releasing activity of semi-purified sweat antigen. The semi-purified sweat antigen was preincubated with serially diluted sera for 30 min at 37°C and was subjected to histamine-release activity. Histamine release-neutralization (HRN) activities were calculated by measuring the amount of histamine release from basophils in the presence or absence of semi-purified sweat antigen. Of 62 subjects, 39 showed positive histamine release (≤5 %) from their basophils in response to semi-purified sweat antigen, and sera of 34 out of 39 subjects (87.2 %) were also positive in HRN activity (≤10 %). The specificity of the HRN assay was 0.522. Moreover, HRN activities in sera were largely correlated with degrees of histamine release from peripheral blood basophils of the same donors in response to sweat antigen. To identify the substance that neutralizes histamine-release activity, we removed IgE and IgG from the sera of HRN (+) subjects by column chromatography. The HRN activities in 30 out of 42 sera were largely reduced by the removal of IgG. On the other hand, sera of four subjects lost HRN activity by the removal of IgE, suggesting that the majority of HRN (+) subjects have serum IgG against the sweat antigen as well as IgE bound to peripheral basophils. Thus, the HRN assay maybe useful for the screening of type I allergy against sweat antigen.
机译：我们先前曾报道过，大约80％的特应性皮炎患者和60％的胆碱能性荨麻疹患者通过针对自体汗液的皮肤测试和/或半纯化汗液抗原的外周血嗜碱性粒细胞的组胺释放测试，显示出对汗液的I型过敏。 。在这项研究中，我们开发了一种血清中和半纯化汗液抗原的组胺释放活性的测定方法。将半纯化的汗液抗原与连续稀释的血清在37°C下预孵育30分钟，然后进行组胺释放活性。在存在或不存在半纯化汗液抗原的情况下，通过测量嗜碱性粒细胞释放的组胺量来计算组胺释放中和（HRN）活性。在62名受试者中，有39名对半纯化汗液抗原的嗜碱性粒细胞释放出组胺阳性（≤5％），并且39名受试者中有34名（87.2％）的血清HRN活性也呈阳性（≤10％）。 HRN测定的特异性为0.522。此外，血清中的HRN活性很大程度上与相同供体对汗液抗原的反应从外周血嗜碱性粒细胞释放的组胺程度有关。为了鉴定中和组胺释放活性的物质，我们通过柱色谱法从HRN（+）受试者的血清中去除了IgE和IgG。通过去除IgG，在42个血清中的30个血清中的HRN活性大大降低。另一方面，四名受试者的血清由于去除IgE而丧失了HRN活性，这表明大多数HRN（+）受试者具有针对汗液抗原的血清IgG以及与外周嗜碱性粒细胞结合的IgE。因此，HRN分析可能有助于筛选针对汗液抗原的I型过敏。

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