Quantitative digital image analysis applied to demonstrate the stratified distribution of involucrin in organ cultured human skin.

Kivinen PK; Hyttinen M; Harvima RJ; Naukkarinen A; Horsmanheimo M; Harvima IT

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Quantitative digital image analysis applied to demonstrate the stratified distribution of involucrin in organ cultured human skin.

机译：定量数字图像分析用于证明在人体培养的器官皮肤中总蛋白的分层分布。

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In this study, quantitative digital image analysis was utilized to measure the optical density of immunostains of involucrin at different depths in the epidermis to obtain reliable ordinal-scaled interpretations of the staining intensity. The distribution of involucrin within the epidermis was investigated in air-liquid interface and submerged skin organ cultures at different time-points. A greyscale calibration procedure to standardize the optical units was used. By the 2nd day of culture, staining of involucrin had shifted markedly towards the mid or basal epidermis. Air-liquid interface cultures showed a less intensive shift than the submerged cultures. Up to the 7th day, involucrin staining remained in the upper epidermis in the air-liquid interface cultures, though weak staining was already observed in the basal epidermis. The results suggest that air-liquid interface conditions maintained physiological conditions better than submerged conditions which result in cultures that may have to increase their involucrin synthesis to improve the barrier function against the surrounding liquid during culture. Alternatively, changes in involucrin synthesis could reflect disturbed homeostasis. Concentrating measurements on certain cell layers might give more detailed information about changes in involucrin expression. Although the detection method was used to study the histochemistry of skin, it could easily be applied to other tissues as well.

机译：在这项研究中，定量的数字图像分析被用来测量表皮中不同深度的总蛋白免疫染色的光密度，以获得对染色强度的可靠的按比例缩放的解释。在不同的时间点，在气液界面和浸没的皮肤器官培养物中研究了表皮中整合素的分布。使用灰度校准程序来标准化光学单元。到培养的第二天，总蛋白的染色已明显移向中表皮或基底表皮。气液界面培养物的转移强度要低于淹没培养物。直到第7天，在气液界面培养中上表皮中仍残留有菊糖蛋白染色，尽管在基底表皮中已经观察到弱染色。结果表明，气液界面条件比淹没条件更好地维持了生理条件，这导致培养物可能必须增加其整合素的合成以改善在培养过程中对周围液体的屏障功能。或者，整合素的合成变化可能反映了稳态的紊乱。在某些细胞层上进行集中测量可能会提供有关整合蛋白表达变化的更多详细信息。尽管该检测方法被用于研究皮肤的组织化学，但是它也可以容易地应用于其他组织。

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《Archives of dermatological research.》 |1999年第4期|共7页
作者
Kivinen PK; Hyttinen M; Harvima RJ; Naukkarinen A; Horsmanheimo M; Harvima IT;
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正文语种 eng
中图分类皮肤病学与性病学;
关键词
Image Processing; Computer-Assisted; Organ Culture; Protein Precursors; Skin; 图像处理; 计算机辅助; 器官培养; 蛋白质前体; 皮肤;

机译：Image Processing;Computer-Assisted;Organ Culture;Protein Precursors;Skin;图像处理;计算机辅助;器官培养;蛋白质前体;皮肤;

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机译：定量数字图像分析用于证明在人体培养的器官皮肤中总蛋白的分层分布。
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Quantitative digital image analysis applied to demonstrate the stratified distribution of involucrin in organ cultured human skin.

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