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首页> 外文期刊>Archives of Insect Biochemistry and Physiology >Biosynthesis and regulation of juvenile hormone III, juvenile hormone III bisepoxide, and methyl farnesoate during the reproductive cycle of the grey fleshfly, Neobellieria (Sarcophaga) bullata.
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Biosynthesis and regulation of juvenile hormone III, juvenile hormone III bisepoxide, and methyl farnesoate during the reproductive cycle of the grey fleshfly, Neobellieria (Sarcophaga) bullata.

机译:在灰蝇蝇新生殖的繁殖周期中,少年激素III,少年激素III双环氧化物和法尼酸甲酯的生物合成和调控。

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To study the effect of brain signals on the biosynthesis of juvenile hormone by the corpora allata of Neobellieria bullata [Sarcophaga bullata], exposed corpora allata connected to the brain were surgically removed from sugar-fed flies and incubatedin vitro with L-[3H-methyl]methionine. After incubation, the media together with the tissues were analysed by HPLC. [3H]Juvenile hormone III (JH III), [3H]JH III bisepoxide (BE), [3H]methyl farnesoate (MF) and an unknown [3H]labelled metabolite (Un) were identified as the primary products. The rate of synthesis of [3H]JH III bisepoxide was higher than that of [3H]JH III, [3H]MF and [3H]Un. Two days after a liver meal, female flies synthesized more JH III, MF, BE and the Un than did males. Synthesis ofJH III, BE and MF in females was lower during the previtellogenic, sugar-feeding period than during the vitellogenic liver-feeding period. Isolated corpus cardiacum-corpus allatum (CC-CA) complexes that were incubated in vitro synthesized less JH III, MFand BE, as compared to complexes that were attached to the brain, indicating that the brain probably modulates the biosynthesis of JH III, MF and BE in the corpora allata. Upon incubation of brain-CC-CA complexes with Neb-TMOF (10-8 M), Neb-colloostatin(10-8 M), ovarian or brain extracts resulted in significant inhibition of JH III and BE biosynthesis in the presence of ovarian extracts. These results indicate that allatostatin-like factors are present in the ovary of the flesh fly.
机译:为了研究脑信号对牛新孢子虫(Sorcophaga bullata)体的生物合成幼体激素的影响,通过手术从食糖果蝇中去除与大脑相连的裸露体,并与L- [3H-甲基蛋氨酸。孵育后,通过HPLC分析培养基和组织。主要产品为[3H]少年激素III(JH III),[3H] JH III双环氧化物(BE),[3H]法尼酸甲酯(MF)和未知的[3H]标记代谢物(Un)。 [3H] JH III双环氧化物的合成速率高于[3H] JH III,[3H] MF和[3H] Un的合成速率。肝餐后两天,雌蝇比雄蝇合成更多的JH III,MF,BE和Un。在雌性前玻璃糖摄食期间,女性的JH III,BE和MF的合成量低于卵黄体肝摄食期间的合成。与附着在大脑上的复合物相比,在体外孵育的离体cardiac体-Allatum(CC-CA)复合物合成的JH III,MF和BE较少,这表明大脑可能调节JH III,MF和BE的生物合成。在主体集中。将脑-CC-CA复合物与Neb-TMOF(10-8 M),Neb-colloostatin(10-8 M)孵育后,卵巢或脑提取物在存在卵巢提取物的情况下会显着抑制JH III和BE的生物合成。这些结果表明在果蝇的卵巢中存在类似阿托伐他汀的因子。

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