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首页> 外文期刊>Applied optics >Influence of the emission-reception geometry in laser-induced fluorescence spectra from turbid media
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Influence of the emission-reception geometry in laser-induced fluorescence spectra from turbid media

机译：发射-接收几何形状对混浊介质激光诱导的荧光光谱的影响

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Routine clinical detection of precancerous lesions by laser-induced autofluorescence was recently demonstrated in several medical fields. This technique is based on the analysis of complex spectra with overlapping broad structures. However, in biological tissues, scattering and absorption are wavelength dependent, and the observed fluorescence signals are distorted when the illumination and detection geometry varies, making comparison of results from different groups difficult. We study this phenomenon experimentally in human tissue in a simple experiment: A fiber is used for the excitation and an identical fiber is used for reception of the signal; both fibers are maintained in contact with the tissue. We study the distortion of the spectra as a function of the distance between the two fibers. For correction of the spectra we show that it is possible to use a fast and accurate ab initio Monte Carlo simulation when the spectral variations of the optical properties of the medium are known. The main advantage of this simulation is its applicability even for complex boundary conditions or when the sample consists of several layers. (C) 1998 Optical Society of America. [References: 30]
机译：最近在几个医学领域证明了通过激光诱导的自发荧光对癌前病变的常规临床检测。该技术基于对具有重叠宽结构的复杂光谱的分析。然而，在生物组织中，散射和吸收取决于波长，并且当照明和检测几何形状变化时，观察到的荧光信号会失真，从而难以比较不同组的结果。我们通过一个简单的实验在人体组织中对这种现象进行了实验研究：光纤用于激发，而相同的光纤用于接收信号;两种纤维都保持与组织接触。我们研究了光谱畸变与两条光纤之间距离的关系。对于光谱的校正，我们表明，当已知介质的光学特性的光谱变化时，可以使用快速准确的从头开始的蒙特卡洛模拟。此模拟的主要优点是，即使在复杂的边界条件下或当样品由多层组成时，它也适用。 （C）1998年美国眼镜学会。 [参考：30]

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