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首页> 外文期刊>Applied optics >FREQUENCY-DOMAIN METHOD FOR MEASURING SPECTRAL PROPERTIES IN MULTIPLE-SCATTERING MEDIA - METHEMOGLOBIN ABSORPTION SPECTRUM IN A TISSUELIKE PHANTOM
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FREQUENCY-DOMAIN METHOD FOR MEASURING SPECTRAL PROPERTIES IN MULTIPLE-SCATTERING MEDIA - METHEMOGLOBIN ABSORPTION SPECTRUM IN A TISSUELIKE PHANTOM

机译：频域法测量多散射介质中的光谱特性-类似于类组织幻影的高铁血红蛋白吸收光谱

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摘要

We have measured the optical absorption and scattering coefficient spectra of a multiple-scattering medium (i.e., a biological tissue-simulating phantom comprising a lipid colloid) containing methemoglobin by using frequency-domain techniques. The methemoglobin absorption spectrum determined in the multiple-scattering medium is in excellent agreement with a corrected methemoglobin absorption spectrum obtained from a steady-state spectrophotometer measurement of the optical density of a minimally scattering medium. The determination of the corrected methemoglobin absorption spectrum takes into account the scattering from impurities in the methemoglobin solution containing no lipid colloid. Frequency-domain techniques allow for the separation of the absorbing from the scattering properties of multiple-scattering media, and these techniques thus provide an absolute measurement of the optical absorption spectra of the methemoglobin/lipid colloid suspension. One accurately determines the absolute methemoglobin absorption spectrum in the frequency domain by extracting the scattering and absorption coefficients from the phase shift Phi and average light intensity DC (or Phi and the amplitude of the light-intensity oscillations AC) data with relationships provided by diffusion theory, but one determines it less accurately by using the Phi and modulation M (M = AC/DC) data and the diffusion theory relationships. In addition to the greater uncertainty in the absorption and scattering coefficients extracted from the Phi and M data, the optical parameters extracted from the Phi and M data exhibit systematically inaccurate behavior that cannot be explained by random noise in the system. Possible reasons for the systematically lower accuracy of the methemoglobin absorption spectrum obtained from Phi and M data are discussed. [References: 35]
机译：我们已经通过使用频域技术测量了含有高铁血红蛋白的多重散射介质（即包含脂质胶体的生物组织模拟体模）的光学吸收和散射系数光谱。在多重散射介质中测定的高铁血红蛋白吸收光谱与从最小散射介质的光密度的稳态分光光度计测量获得的校正高铁血红蛋白吸收光谱非常一致。校正的高铁血红蛋白吸收光谱的确定考虑了不包含脂质胶体的高铁血红蛋白溶液中杂质的散射。频域技术可将吸收与多种散射介质的散射特性区分开，因此这些技术可提供对高铁血红蛋白/脂质胶体悬浮液的光吸收光谱的绝对测量。通过扩散理论提供的关系，通过从相移Phi和平均光强度DC（或Phi和光强度振荡AC的振幅）数据中提取散射和吸收系数，可以准确地确定频域中的高铁血红蛋白吸收光谱，但是使用Phi和调制M（M = AC / DC）数据以及扩散理论关系来确定精度较低。除了从Phi和M数据中提取的吸收和散射系数存在较大不确定性之外，从Phi和M数据中提取的光学参数还表现出系统上不准确的行为，无法用系统中的随机噪声来解释。讨论了从Phi和M数据获得的高铁血红蛋白吸收光谱的系统准确性较低的可能原因。 [参考：35]

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