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Spatial distribution of fluorescence intensity within large droplets and its dependence on dye concentration

机译：大液滴内荧光强度的空间分布及其对染料浓度的依赖性

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摘要

The dependence of fluorescence intensity distributions within droplets on added dye concentration has been calculated by extension of the geometrical-optics approximation and verified by experimental observations. With rising dye concentration, surface plots of the equatorial fluorescence pattern show decreasing relevance of intensity enhancement at focusing points of internal light rays and increasing effects of linear absorption on the characteristic features of the distribution. For comparison with experimentally obtained images of the fluorescence intensity distribution within droplets, a method for calculating volume-integrated intensity distributions was developed in which image distortion at the fluid-air interface is included. A comparison of the calculated and the experimentally determined fluorescence intensity distributions within a droplet confirmed the accuracy of the geometrical-optics approach at high dye concentrations. However, discrepancies from experimental results are visible at low dye concentrations owing to nonlinear optical effects.
机译：通过几何光学近似的扩展计算出液滴内荧光强度分布对所添加染料浓度的依赖性，并通过实验观察进行了验证。随着染料浓度的升高，赤道荧光图案的表面图显示出内部光线聚焦点强度增强的相关性降低，线性吸收对分布特征的影响增加。为了与通过实验获得的液滴内荧光强度分布图像进行比较，开发了一种计算体积积分强度分布的方法，其中包括了流体-空气界面处的图像失真。对液滴内计算的和实验确定的荧光强度分布的比较证实了在高染料浓度下几何光学方法的准确性。但是，由于非线性光学效应，在低染料浓度下可以看到实验结果的差异。

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