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Separating the fluorescence and reflectance components of coral spectra

机译：分离珊瑚光谱的荧光和反射成分

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The optical signal leaving the surface of fluorescing corals is a combination of elastic and inelastic scatter. A new experimental method was developed to separate the fluorescence and the reflectance components that involves measurements with and without a long-pass cutoff filter that eliminates the fluorescence contribution to the signal. The required measurements were performed underwater to demonstrate the applicability of the method for in situ applications. Computations with prototype rather than individually measured fluorescence emission spectra do not significantly compromise the accuracy of the results. A model was developed for calculating the interaction of the reflectance and the fluorescence components with new incident illumination conditions. The model calculations were supported by field experiment. We show that the contribution of fluorescence to some coral's spectra in various illumination conditions justifies consideration in optical models. The results are applicable to modeling the spectra of fluorescing corals under any irradiance spectrum and interpreting remote-sensing data in the relevant wave-length range. Further research is necessary to examine the significance of fluorescence near coral reefs at various scales.
机译：离开发荧光珊瑚表面的光信号是弹性和非弹性散射的组合。开发了一种新的实验方法来分离荧光和反射率分量，这涉及在有和没有长通截止滤波器的情况下进行测量，从而消除了荧光对信号的影响。所需的测量在水下进行，以证明该方法在现场应用中的适用性。用原型而不是单独测量的荧光发射光谱进行计算不会显着影响结果的准确性。开发了用于计算反射率和荧光成分与新的入射照明条件之间的相互作用的模型。模型计算得到现场实验的支持。我们表明，在各种光照条件下荧光对某些珊瑚光谱的贡献证明了在光学模型中的考虑。该结果可用于对任何辐照光谱下的发荧光珊瑚光谱进行建模，并解释相关波长范围内的遥感数据。有必要进行进一步的研究以检查不同尺度下珊瑚礁附近荧光的重要性。

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