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Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms

机译:菲涅耳离轴全息图的数值重建同时振幅对比和定量相位对比显微镜

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摘要

We present a digital method for holographic microscopy involving a CCD camera as a recording device. Off-axis holograms recorded with a magnified image of microscopic objects are numerically reconstructed in amplitude and phase by calculation of scalar diffraction in the Fresnel approximation. For phasecontrast imaging the reconstruction method involves the computation of a digital replica of the reference wave. A digital method for the correction of the phase aberrations is presented. We present a detailed description of the reconstruction procedure and show that the transverse resolution is equal to the diffraction limit of the imaging system.
机译:我们提出了一种将CCD相机作为记录设备的全息显微术的数字方法。通过在菲涅耳近似中计算标量衍射,可以在振幅和相位上数值重建以微观对象放大图像记录的离轴全息图。对于相衬成像,重建方法包括计算参考波的数字副本。提出了一种校正相差的数字方法。我们提出了重建程序的详细说明,并表明横向分辨率等于成像系统的衍射极限。

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