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Light scattering from cells: finite-difference time-domain simulations and goniometric measurements

机译:来自细胞的光散射:时域有限差分模拟和角度测量

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摘要

We have examined the Iight-scattering properties ofinhomogeneous biological cells through a combination of theoretical simulations and goniometric measurements. A finite-difference time-domain (FDTD) technique was used to compute intensity as a function of scattering angle for cells containing multiple organelles and spatially varying index of refraction profiles. An automated goniometer was constructed to measure the scattering properties ofdilute cell suspensions. Measurements compared favorably with FDTD predictions. FDTD and experimental results indicate that scattering properties are strongly infiuenced by cellular biochemical and morphoIogical structure.
机译:我们已经通过理论模拟和测角测量相结合的方式检查了不均匀生物细胞的光散射特性。有限差分时域(FDTD)技术用于计算强度,该强度是包含多个细胞器和空间变化的折射率分布的细胞的散射角的函数。构造了自动测角仪以测量稀细胞悬液的散射特性。与FDTD预测相比,测量结果令人满意。 FDTD和实验结果表明,细胞的生化和形态结构对散射特性影响很大。

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