首页> 外文期刊>Applied optics >Collection of fluorescence from single molecules in microspheres: Effects of illumination geometry
【24h】

Collection of fluorescence from single molecules in microspheres: Effects of illumination geometry

机译:微球中单个分子的荧光收集:照明几何形状的影响

获取原文
获取原文并翻译 | 示例
           

摘要

The collection of fluorescence from a molecule inside a sphere illuminated with single or counterpropagating plane waves is modeled. The results are applicable to microdroplet-based single molecule detection techniques and to some microparticle characterization techniques using inelastic emission. The large position-dependent variations in the fluorescence collection rate are primarily attributable to variations in the excitation intensity. With plane-wave illumination the collection from shadow regions is low because the incident energy is refracted by the droplet surface away from these regions. The average collection rate from molecules in shadow regions can be increased by illuminating with counterpropagating beams. (C) 1997 Optical Society of America.
机译:对来自单个或反向传播的平面波照射的球体内分子的荧光集合进行建模。该结果适用于基于微滴的单分子检测技术以及某些使用非弹性发射的微粒表征技术。荧光收集速率中与位置有关的较大变化主要归因于激发强度的变化。在平面波照明下,阴影区域的收集量较低,因为入射能量会被远离这些区域的液滴表面折射。通过用反向传播的光束照射,可以提高阴影区域分子的平均收集率。 (C)1997年美国眼镜学会。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号