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首页> 外文期刊>Applied optics >ELASTIC LIGHT-SCATTERING MEASUREMENTS OF SINGLE BIOLOGICAL CELLS IN AN OPTICAL TRAP
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ELASTIC LIGHT-SCATTERING MEASUREMENTS OF SINGLE BIOLOGICAL CELLS IN AN OPTICAL TRAP

机译：光学陷阱中单个生物细胞的弹性散射光测量

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We have developed an instrument for determination of the angular light scattering of beads and biological cells. The instrument uses radiation pressure for levitation of particles inside a cuvette. The setup consists of two 780-nm diode lasers in a vertical double-beam trapping configuration. In the horizontal direction a weakly focused 633-nm probe beam is used to illuminate the trapped particle. One can detect scattered light over the range of from -150 to 150 deg with an angular resolution of 0.9 deg using an avalanche photodiode. With this setup light scattering from polystyrene beads was measured, and the obtained scattering patterns were compared with theoretical scattering patterns from Lorenz-Mie theory. The results show that the setup is stable, gives reproducible patterns, and qualitatively agrees with the calculations. Trapping of biological cells is more difficult than trapping of beads, because smaller forces result from smaller refractive indices. We present an angular scattering pattern measured from a human lymphocyte measured from 20 to 60 deg. (C) 1996 Optical Society of America [References: 37]
机译：我们已经开发出一种用于确定珠子和生物细胞的角光散射的仪器。该仪器使用辐射压力来悬浮比色杯中的颗粒。该设置包括两个780 nm二极管激光器，它们均为垂直双束陷波配置。在水平方向上，弱聚焦的633 nm探测光束用于照亮捕获的粒子。使用雪崩光电二极管可以检测到-150至150度范围内的散射光，其角分辨率为0.9度。通过这种设置，测量了来自聚苯乙烯珠的光散射，并将获得的散射图与来自Lorenz-Mie理论的理论散射图进行了比较。结果表明，该装置稳定，给出了可重现的图案，并且定性地与计算结果吻合。捕获生物细胞比捕获珠子更困难，因为较小的力来自较小的折射率。我们提出了从20至60度的人类淋巴细胞测量的角度散射模式。 （C）1996年美国眼镜学会[参考文献：37]

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