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SINGLE-MOLECULE DETECTION - APPLICATIONS TO ULTRASENSITIVE BIOCHEMICAL ANALYSIS

机译:单分子检测-在超灵敏生物化学分析中的应用

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Recent developments in laser-based detection of fluorescent molecules have made possible the implementation of very sensitive techniques for biochemical analysis. We present and discuss our experiments on the applications of our recently developed technique of single-molecule detection to the analysis of molecules of biological interest. These newly developed methods are capable of detecting and identifying biomolecules at the single-molecule level of sensitivity. In one case, identification is based on measuring fluorescence brightness from single molecules. In another, molecules are classified by determining their electrophoretic velocities. [References: 16]
机译:基于激光的荧光分子检测技术的最新发展已使非常敏感的生物化学分析技术得以实现。我们提出并讨论了我们最近开发的单分子检测技术在分析具有生物学意义的分子中的应用的实验。这些新开发的方法能够以单分子敏感性水平检测和鉴定生物分子。在一种情况下,鉴定是基于测量单个分子的荧光亮度。在另一种方法中,分子通过确定其电泳速度进行分类。 [参考:16]

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