...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied in vitro toxicology. >An Integrative Proteomic/Lipidomic Analysis of the Gold Nanoparticle Biocorona in Healthy and Obese Conditions
【24h】

An Integrative Proteomic/Lipidomic Analysis of the Gold Nanoparticle Biocorona in Healthy and Obese Conditions

机译：一个整合蛋白质组学/ Lipidomic分析黄金纳米粒子Biocorona健康和肥胖条件

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Introduction: When nanoparticles (NPs) enter a physiological environment, a coating of biomolecules or biocorona (BC) forms on the surface. Formation of the NP-BC is dependent on NP properties, the physiological environment, and time. The BC influences NP properties and biological interactions such as cellular internalization, immune responses, biodistribution, and others, leading to pharmacological and toxicological consequences. To date, examination of the NP-BC has focused primarily on protein components and healthy conditions. Therefore, we evaluated the protein and lipid content of BCs that formed on physicochemically distinct gold nanoparticles (AuNPs) under healthy and obese conditions. A comprehensive understanding of the NP-BC is necessary for the translation of in vitro toxicity assessments to clinical applications.Materials and Methods: AuNPs with two coatings (poly-N-vinylpyrrolidone [PVP] or citrate) and diameters (20 or 100?nm) were incubated in pooled human serum, and an integrated proteomic/lipidomic approach was used to evaluate BC composition. Macrophages were utilized to evaluate differential immune responses due to variations in the AuNP-BC.Results: AuNPs form distinct BCs based on physicochemical properties and the surrounding environment, with the obese BC containing more proteins and fewer lipids than the healthy BC. Differential macrophage inflammatory responses were observed based on AuNP properties and BC composition.Discussion and Conclusion: Overall, these findings demonstrate that AuNP size and coating, as well as physiological environment, influence the protein and lipid composition of the BC, which impacts cellular responses following exposure. These findings demonstrate that incorporation of BCs representing distinct physiological conditions may enhance the translatability of nanosafety in vitro studies.
机译：简介:当纳米粒子(NPs)输入涂层的生理环境生物分子或biocorona (BC)的形式表面。NP属性,生理环境,时间。细胞等生物的相互作用内化,免疫反应,biodistribution等等,导致药理学和毒理学的后果。到目前为止,NP-BC集中检查主要蛋白质成分和健康条件。和BCs形成的脂质含量物理化学的金纳米粒子在健康和肥胖的条件下(AuNPs)。全面了解NP-BC体外翻译所必需的临床毒性评估应用程序。两个涂层(poly-N-vinylpyrrolidone (PVP)或柠檬酸)和直径(20或100 ?海里)在孵化池人类血清和一个蛋白质组学/ lipidomic方法综合使用公元前评价组成。利用评估微分免疫由于变化的反应AuNP-BC。物理化学性质和周围环境,与肥胖BC包含更多蛋白质和脂质少于健康BC。微分巨噬细胞炎症反应基于AuNP属性和BC观察组成。这些发现表明AuNP大小和涂料,以及生理环境,影响蛋白质和脂类组成公元前,影响细胞反应后曝光。BCs代表不同的整合生理条件可能增强nanosafety体外研究的可译性。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号