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首页> 外文期刊>Brazilian journal of chemical engineering >COMPARISON BETWEEN CONTINUOUS AND BATCH PROCESSING TO PRODUCE XYLANASE BY Penicillium canescens 10-10c
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COMPARISON BETWEEN CONTINUOUS AND BATCH PROCESSING TO PRODUCE XYLANASE BY Penicillium canescens 10-10c

机译：棒状青霉10-10c连续法和间歇法生产木聚糖酶的比较

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摘要

Penicillium canescens 10-10c strain was cultivated on barley straw hydrolysate as a soluble nutrient source and as inducer for xylanase production. Barley straw hydrolysate was obtained by treatment of barley straw with NaOH or hot water. In shake flask cultures, NaOH treatment was found to increase the biomass production, but was not accompanied by an increase in xylanase production. The best xylanase production (54 U/ml) was observed on hydrolyzed extract from barley straw treated with hot water (100 °C) for 3 hours. Enzyme production was further improved by scaling up the cultivation process to a 3-L stirred tank bioreactor. For batch cultivations in the bioreactor, the maximum xylanase productivity reached 1.31 and 0.46 U/ml/h, respectively, after 96 and 168 hours of cultivation. However, xylanase productivity reached 3.46 U/ml/h in the continuous culture. These results suggest that xylanase can be produced efficiently by Penicillium canescens 10-10c in continuous culture from an inexpensive source such as barley straw hydrolysate.
机译：在大麦秸秆水解产物上培养甘蔗青霉10-10c菌株作为可溶性营养源和木聚糖酶产生的诱导剂。通过用NaOH或热水处理大麦草获得大麦草水解物。在摇瓶培养中，发现用NaOH处理可增加生物量的产生，但不会伴随木聚糖酶产生的增加。在用热水（100°C）处理3小时的大麦秸秆的水解提取物中观察到最佳的木聚糖酶产量（54 U / ml）。通过将培养过程扩大到3升搅拌釜生物反应器，可以进一步提高酶的产量。对于生物反应器中的分批培养，在96和168小时的培养后，最大的木聚糖酶生产率分别达到1.31和0.46 U / ml / h。然而，在连续培养中木聚糖酶生产率达到3.46U / ml / h。这些结果表明，甘蔗青霉10-10c可以在廉价的来源如大麦秸秆水解产物的连续培养中有效地产生木聚糖酶。

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