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首页> 外文期刊>Biology of Reproduction: Offical Journal of the Society for the Study of Reproduction >Birth of pouch young after artificial insemination in the tammar wallaby (Macropus eugenii)
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Birth of pouch young after artificial insemination in the tammar wallaby (Macropus eugenii)

机译：人工授精后袋年轻的诞生在tammar小袋鼠(捕食eugenii)

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摘要

Timing of artificial insemination (AI) in marsupials is critical because fertilization must occur before mucin coats the oocyte during passage through the oviduct. In this study, timing and the site of insemination were examined to develop AI in the tammar wallaby (Macropus eugenii). Birth and postpartum (p.p.) estrus was synchronized in 46 females. Epididymal spermatozoa (n = 4) or semen collected by electroejaculation (n = 42) were inseminated early (4-21 h p.p.) into the urogenital sinus (n = 7), the anterior vaginal culs de sac (n = 7), the uterus by transcervical catheter (n = 5), or the uterus by injection (intrauterine artificial insemination, IUAI) (n = 5). A further 16 females were inseminated late (19-48 h p.p.) by IUAI. All females were monitored for birth. A third group of six females was inseminated late (21-54 h p.p.) by IUAI and 0.4-6.6 h later, sperm had reached the oviduct in all animals. In total, an oocyte to which spermatozoa were attached was recovered and two young were born after IUAI using epididymal (n = 1) or electroejaculated (n = 2) spermatozoa, but no young resulted from insemination at other sites. Two females were successfully inseminated at 43 and 47 h p.p., later than most other animals, and the third was inseminated much earlier (18 h p.p.) but with highly motile spermatozoa. These young represent the first macropodids born by AI and the first marsupials conceived using epididymal spermatozoa.
机译：人工授精技术(AI)的时机有袋动物因为受精必须是至关重要的粘蛋白层卵母细胞在之前发生通过输卵管。时间和地点受精检查发展人工智能的tammar小袋鼠(捕食eugenii)。同步在46岁女性。精子(n = 4)或收集的精液electroejaculation (n = 42)受精早期(4-21 h p.p。)泌尿生殖窦(n= 7),前阴道cul de囊(n = 7),的子宫transcervical导管(n = 5),或注射子宫宫内人工受精,IUAI) (n = 5)。16岁女性在受精(由IUAI 19-48 h p.p。)。女性被监控。6雌性受精迟到(21-54 h由IUAI p.p。),0.4 - -6.6 h后,精子在所有动物到达输卵管。卵母细胞,精子被附加恢复和两个年幼的IUAI后出生使用附睾的(n = 1)或electroejaculated (n= 2)精子,但不年轻了受精在其他网站。成功在43和47 h p.p。人工受精,比大多数其他动物之后,第三个受精(18 h p.p。),但更早高活力的精子。第一个macropodids AI和第一个出生的有袋动物使用附睾的构思精子。

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