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首页> 外文期刊>Archives of Environmental Contamination and Toxicology >Comparison of bioluminescent dinoflagellate (QwikLite) and bacterial (Microtox) rapid bioassays for the detection of metal and ammonia toxicity.
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Comparison of bioluminescent dinoflagellate (QwikLite) and bacterial (Microtox) rapid bioassays for the detection of metal and ammonia toxicity.

机译：比较生物发光的鞭毛藻（QwikLite）和细菌（Microtox）快速生物测定法检测金属和氨的毒性。

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摘要

This study compared the sensitivity of two rapid toxicity tests, QwikLite and Microtox, to seven metals and ammonia. Both of these tests measure a reduction in light production from bioluminescent microorganisms (dinoflagellates and marine bacteria, respectively) as a means of toxicity detection and are simple and inexpensive to conduct compared to many standardized acute toxicity tests. For QwikLite tests, three marine dinoflagellate species (Lingulodinium polyedrum, Ceratocorys horrida, and Pyrocystis noctiluca) were separately evaluated following a 24-h exposure period. The marine bacterium, Vibrio fischeri, was used in the Microtox tests, in 15-min exposures to the same metal preparations as those used for the QwikLite tests. The QwikLite tests were generally one to two orders of magnitude more sensitive than the Microtox tests, as indicated by lower median effects concentrations (EC(50)). Both QwikLite and Microtox, however, resulted in similar toxicity rankings for the metals tested. The dinoflagellate species used in the QwikLite tests responded similarly for most compounds tested, with L. polyedrum appearing to be somewhat more sensitive than the other two species for most metals evaluated. QwikLite was also more comparable in sensitivity to several commonly used standardized toxicity tests. As with all toxicity tests, species selection for QwikLite should take into account study-specific factors, including the potential for sensitivity to confounding factors, such as ammonia.
机译：这项研究比较了两种快速毒性试验QwikLite和Microtox对7种金属和氨的敏感性。这两种测试都测量了生物发光微生物（分别为鞭毛藻和海洋细菌）产生的光的减少，以此作为毒性检测的手段，并且与许多标准化的急性毒性测试相比，其操作简便，成本低廉。对于QwikLite测试，在暴露24小时后，分别评估了三种海洋鞭毛藻种类（灵芝，多角藻，夜蛾热囊藻）。在Microtox测试中使用了海洋细菌费氏弧菌，将其暴露在与QwikLite测试所用的相同金属制剂中15分钟。 QwikLite测试的敏感性通常比Microtox测试高一到两个数量级，这表明较低的中位效应浓度（EC（50））。然而，QwikLite和Microtox对被测金属的毒性排名均相似。 QwikLite测试中使用的鞭毛藻物种对大多数测试化合物的反应相似，对于大多数所评估的金属而言，多齿乳杆菌似乎比其他两种物种敏感。 QwikLite在灵敏度上也可以与几种常用的标准化毒性试验相比。与所有毒性测试一样，QwikLite的物种选择应考虑特定研究因素，包括对混杂因素（例如氨）的敏感性。

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