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Real-time monitoring of cyclic nucleotide signaling in neurons using genetically encoded FRET probes.

机译:使用遗传编码的FRET探针实时监测神经元中的环状核苷酸信号。

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Signaling cascades involving cyclic nucleotides play key roles in signal transduction in virtually all cell types. Elucidation of the spatiotemporal regulation of cyclic nucleotide signaling requires methods for tracking the dynamics of cyclic nucleotides and the activities of their regulators and effectors in the native biological context. Here we review a series of genetically encoded FRET-based probes for real-time monitoring of cyclic nucleotide signaling with a particular focus on their implementation in neurons. Current data indicate that neurons have a very active metabolism in cyclic nucleotide signaling, which is tightly regulated through a variety of homeostatic regulations.
机译:涉及环核苷酸的信号级联实际上在所有细胞类型中都在信号转导中起关键作用。阐明环核苷酸信号的时空调节需要在自然生物学环境中跟踪环核苷酸动态及其调节剂和效应子活性的方法。在这里,我们回顾了一系列基于基因编码的基于FRET的探针,用于实时监测环核苷酸信号,特别关注它们在神经元中的实现。当前数据表明,神经元在环状核苷酸信号传导中具有非常活跃的新陈代谢,该信号通过各种体内平衡调节受到严格调节。

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