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首页> 外文期刊>Archives of microbiology >THE ROLE OF AN NAD-INDEPENDENT LACTATE DEHYDROGENASE AND ACETATE IN THE UTILIZATION OF LACTATE BY CLOSTRIDIUM ACETOBUTYLICUM STRAIN P262
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THE ROLE OF AN NAD-INDEPENDENT LACTATE DEHYDROGENASE AND ACETATE IN THE UTILIZATION OF LACTATE BY CLOSTRIDIUM ACETOBUTYLICUM STRAIN P262

机译：NAD无关的乳酸脱氢酶和醋酸盐在乙酰丁酸梭菌菌株P262利用乳酸中的作用

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Clostridium acetobutylicum strain P262 utilized lactate at a rapid rate [600nmol min(-1) (mg protein)(-1)], but lactate could not serve as the sole energy source. When acetate was provided as a co-substrate, the growth rate was 0.05 h(-1). Butyrate, carbon dioxide and hydrogen were the end products of lactate and acetate utilization, and the stoichiometry was 1 lactate + 0.4 acetate --> 0.7 butyrate + 0.6 H-2 + 1 CO2. Lactate-grown cells had twofold lower hydrogenase than glucose-grown cells, and the lactate-grown cells used acetate as an alternative electron acceptor. The cells had a poor affinity for lactate (K-s = 1.1 mM), and there was no evidence for active transport. Lactate utilization was catabolyzed by an inducible NAD-independent lactate dehydrogenase (iLDH) that had a pH optimum of 7.5. The iLDH was fivefold more active with D-lactate than L-lactate, and the K-m for D-lactate was 3.2 mM. Lactate-grown cells had little butyraldehyde dehydrogenase activity, and this defect did not allow the conversion of lactate to butanol. [References: 31]
机译：丙酮丁醇梭菌菌株P262快速利用乳酸[600nmol min（-1）（mg蛋白）（-1）]，但乳酸不能作为唯一的能源。当提供乙酸盐作为共底物时，生长速率为0.05 h（-1）。丁酸盐，二氧化碳和氢气是乳酸和乙酸盐利用的最终产物，化学计量为1乳酸+ 0.4乙酸盐-> 0.7丁酸盐+ 0.6 H-2 + 1 CO2。乳酸生长的细胞的氢化酶比葡萄糖生长的细胞低两倍，而乳酸生长的细胞使用乙酸盐作为替代电子受体。这些细胞对乳酸的亲和力很弱（K-s = 1.1 mM），并且没有主动转运的证据。乳酸的利用被可诱导的非NAD依赖性乳酸脱氢酶（iLDH）催化，其pH最适值为7.5。 iLDH与D-乳酸的活性是L-乳酸的五倍，D-乳酸的K-m为3.2 mM。乳酸生长的细胞几乎没有丁醛脱氢酶活性，这种缺陷不允许乳酸转化为丁醇。 [参考：31]

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