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首页> 外文期刊>Archives of microbiology >IMMUNO-GOLD LOCALIZATION OF HEPATOTOXINS IN CYANOBACTERIAL CELLS
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IMMUNO-GOLD LOCALIZATION OF HEPATOTOXINS IN CYANOBACTERIAL CELLS

机译：蓝藻细胞中肝毒素的免疫金定位

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A polyclonal antibody against the potent hepatotoxic cyclic peptide microcystins and nodularins was used in conjunction with immune-gold labelling to localize the toxins in three strains of cyanobacteria. Ultrastructurally, there were no major differences between unicellular Microcystis aeruginosa strain PCC 7820 (toxin-producing strain) and M. aeruginosa strain UTEX 2063 (nontoxin-producing strain), except that M. aeruginosa PCC 7820 cells had a sheath. The thickness of the sheath was about 12 nm and was distinguishable from the cell wall at the ultrastructural level only when the specimen was stained en bloc with uranyl acetate. Microcystins and nodularin were found in M. aeruginosa PCC 7820 and Nodularia spumigena strain L-575 respectively, but not in nontoxic M. aeroginosa UTEX 2063. In M. aeruginosa PCC 7820 cells, microcystin was found primarily in the thylakoid area and nucleoid, with smaller amounts in the cell wall and sheath. Only nonspecific labelling was found in other cellular inclusions, such as polyhedral bodies, cyanophycin granules and membrane-limited inclusions. In strain N. spumigena L-575, nodularin was found in both vegetative cells and heterocysts with a distribution similar to that in M. aeruginosa PCC 7820. [References: 27]
机译：针对强肝毒性环肽微囊藻毒素和结节菌素的多克隆抗体与免疫金标记结合使用，可将毒素定位在三种蓝细菌菌株中。在超微结构上，除了铜绿假单胞菌PCC 7820细胞有鞘外，单细胞铜绿假单胞菌PCC 7820（产毒菌株）和铜绿假单胞菌菌株UTEX 2063（非产毒菌株）之间没有主要差异。鞘的厚度为约12nm，并且仅当样品被乙酸铀酰整体染色时才在超微结构水平上与细胞壁区分开。微囊藻毒素和结节菌素分别在铜绿假单胞菌PCC 7820和散发性结节杆菌L-575菌株中发现，但在无毒铜绿假单胞菌UTEX 2063中未发现。在铜绿假单胞菌PCC 7820细胞中，微囊藻毒素主要存在于类囊体和核样中。细胞壁和鞘中的数量较少。在其他细胞包裹体中，例如多面体，蓝霉素颗粒和膜受限包裹体中，仅发现非特异性标记。在spumigena N. L-575菌株中，在营养细胞和异质囊中均发现了结节菌素，其分布与绿脓杆菌PCC 7820相似。[参考文献：27]

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