首页> 外文期刊>Archives of microbiology >GLUTAMATE EXCRETION IN ESCHERICHIA COLI - DEPENDENCY ON THE RELA AND SPOT GENOTYPE
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GLUTAMATE EXCRETION IN ESCHERICHIA COLI - DEPENDENCY ON THE RELA AND SPOT GENOTYPE

机译:大肠杆菌中谷氨酸的分泌与RELA和点基因型的关系。

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Glutamate excretion due to amino acid starvation was investigated in ''stringent'' and ''relaxed'' strains of Escherichia coli. The observed excretion process is relA-dependent, carrier-mediated, and glutamate-specific. After induction, excretion was detected within less than 2 min and continued for more than 5 h with a rate of 7-10 nmol (mg dry weight)(-1) mim(-1). Using carbonyl cyanide m-chlorophenylhydrazone or polymyxin B nonapeptide, together with valinomycin, it was shown that glutamate excretion is driven by the membrane potential. [References: 18]
机译:在大肠杆菌的“严格”和“松弛”菌株中研究了由于氨基酸饥饿导致的谷氨酸排泄。观察到的排泄过程是relA依赖性的,载体介导的和谷氨酸特异性的。诱导后,在不到2分钟的时间内检测到排泄物,并以7-10 nmol(毫克干重)(-1)mim(-1)的速率持续超过5小时。使用羰基氰化物间氯苯基hydr或多粘菌素B九肽与缬氨霉素一起显示,谷氨酸排泄是由膜电位驱动的。 [参考:18]

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