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首页> 外文期刊>Archives of microbiology >Gene expression modulation by chalcopyrite and bornite in Acidithiobacillus ferrooxidans.
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Gene expression modulation by chalcopyrite and bornite in Acidithiobacillus ferrooxidans.

机译：在酸性氧化铁硫杆菌中由黄铜矿和斑铜矿调节基因表达。

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Acidithiobacillus ferrooxidans is a mesophilic, acidophilic, chemolithoautotrophic bacterium that obtains energy from the oxidation of ferrous iron (Fe2+), elemental sulfur and reduced sulfur compounds. The industrial interest in A. ferrooxidans resides in its capacity to oxidize insoluble metal sulfides into soluble metal sulfates, thus allowing the recovery of the desired metals from low-grade sulfide ores. In the present work, RNA arbitrarily primed PCR (RAP-PCR) was performed to identify cDNAs differentially expressed in A. ferrooxidans cells grown in the presence of Fe2+ and cells maintained for 24 h in the presence of the copper sulfides bornite and chalcopyrite. Eighteen cDNAs corresponding to genes with known function were identified, and their relative expression was further characterized by real-time quantitative PCR. Bornite had a mild effect on the expression of the 18 genes analyzed. None of these genes was down-regulated and among the few genes up-regulated, it is worth mentioning lepA and def-2 that are involved in protein synthesis. Chalcopyrite presented the most significant changes. Five genes related to protein processing were down-regulated, and another 5 genes related to the transport system were up-regulated. The up- and down-regulation of these genes in the presence of bornite and chalcopyrite could be due to alterations in the ideal pH, presence of copper ions in solution and nutrient limitation. The results suggest that gene expression modulation might be important for the A. ferrooxidans early response to copper sulfides.
机译：酸性氧化硫硫杆菌是一种嗜温，嗜酸性，化石自养细菌，可通过氧化亚铁（Fe2 +），元素硫和还原的硫化合物获得能量。铁氧化农杆菌的工业利益在于其将不溶性金属硫化物氧化成可溶性金属硫酸盐的能力，从而允许从低品位硫化物矿石中回收所需金属。在目前的工作中，进行了RNA任意引物PCR（RAP-PCR），以鉴定在Fe2 +存在下生长的氧化铁农杆菌细胞中以及在硫化铜铜矿和黄铜矿存在下保持24小时的细胞中差异表达的cDNA。鉴定出18个与已知功能基因相对应的cDNA，并通过实时定量PCR进一步表征了它们的相对表达。硼铁矿对所分析的18个基因的表达有轻微的影响。这些基因没有一个被下调，在少数几个上调的基因中，值得一提的是参与蛋白质合成的lepA和def-2。黄铜矿呈现出最显着的变化。与蛋白质加工有关的五个基因被下调，与运输系统有关的另外五个基因被上调。在钙铁矿和黄铜矿存在下这些基因的上调和下调可能是由于理想pH值的变化，溶液中铜离子的存在和营养限制所致。结果表明基因表达调节可能对A.ferrooxidans对硫化铜的早期反应很重要。

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