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首页> 外文期刊>Archives of microbiology >The UvrY response regulator of the BarA-UvrY two-component system contributes to Yersinia ruckeri infection of rainbow trout (Oncorhynchus mykiss).
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The UvrY response regulator of the BarA-UvrY two-component system contributes to Yersinia ruckeri infection of rainbow trout (Oncorhynchus mykiss).

机译：BarA-UvrY两组分系统的UvrY响应调节剂促成了彩虹鳟鱼（Oncorhynchus mykiss）的耶尔森氏菌Ruckeri感染。

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To identify virulence-associated genes of a fish pathogen Yersinia ruckeri, we screened a total of 1056 mini-Tn5-Km2 signature-tagged mutants in rainbow trout by immersion challenge. Of 1056, 25 mutants were found survival-defective as they could not be re-isolated from fish kidney 7 days after infection. Mutated gene in F2-4 mutant, one of the 25 mutants, was homologous to uvrY that encodes UvrY response regulator of BarA-UvrY two-component system (TCS). Mutant F2-4 was significantly more sensitive (P < 0.05) to H2O2-mediated killing and was less able to infect Epithelioma papulosum cyprini cells. However, UvrY mutation did not affect survival of F2-4 mutant in the presence of non-immune fish serum and its ability to grow under iron starvation. In a time-course co-infection, mutant F2-4 had lower bacterial loads on day 1 itself, and by day 5 there was nearly a 1,000-fold difference in infection levels of the parent and mutant strains. The barA homolog of Y. ruckeri was PCR-amplified and sequence analyses identified four domains that were characteristic of hybrid histidine kinases. To conclude, the BarA-UvrY TCS contributes to the pathogenesis of Y. ruckeri in its natural host rainbow trout, possibly by regulating invasion of epithelial cells and sensitivity to oxidative stress induced by immune cells.
机译：为了鉴定鱼类病原体耶尔森氏菌的毒力相关基因，我们通过浸没攻击在虹鳟鱼中筛选了总共1056个mini-Tn5-Km2标记标签的突变体。在1056个中，发现25个突变体具有生存缺陷，因为它们在感染后7天无法从鱼肾中重新分离出来。 F2-4突变体（25个突变体之一）中的突变基因与uvrY同源，该蛋白编码BarA-UvrY两组分系统（TCS）的UvrY反应调节因子。 F2-4突变体对H2O2介导的杀伤作用更敏感（P <0.05），并且感染鲤鱼上皮细胞的能力较弱。但是，UvrY突变不会影响F2-4突变体在非免疫鱼血清的存在下的存活率及其在铁饥饿状态下的生长能力。在时程共感染中，突变体F2-4在第1天本身的细菌载量较低，而到第5天，亲本和突变菌株的感染水平相差近1,000倍。 PCR扩增ruckeri。的barA同源物，并且序列分析鉴定了四个具有组氨酸组氨酸激酶特征的结构域。总而言之，BarA-UvrY TCS可能通过调节上皮细胞的侵袭和对免疫细胞诱导的氧化应激的敏感性，在其自然宿主虹鳟中促进了R. ruckeri的发病。

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