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首页> 外文期刊>Archives of microbiology >Culture conditions affecting biodegradation components of the brown-rot fungus Gloeophyllum trabeum.
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Culture conditions affecting biodegradation components of the brown-rot fungus Gloeophyllum trabeum.

机译：培养条件会影响褐腐真菌Gloeophyllum trabeum的生物降解成分。

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摘要

To determine the liquid culture conditions under which the wood-degrading system of the brown-rot fungus Gloeophyllum trabeum is expressed, enzymes and metabolites from liquid and solid substrate cultures were characterized. Enzymes were analyzed by 2-D gel electrophoresis and also assayed. Growth conditions were varied by using liquid media containing: (1) low carbon, low nitrogen, (2) low carbon, high nitrogen, (3) high carbon, low nitrogen, or (4) high carbon, high nitrogen. The protein arrays expressed under the four conditions were very similar, and endo-1,4-beta-glucanase (detected by 2-D gels) activity along with beta-glucosidase, xylanase, and NADH/quinone oxidoreductase activities were detected. Maximal expression of the hydrolytic enzymes was observed in high carbon/high nitrogen medium, whereas the highest oxidoreductase activity was in the high carbon low nitrogen medium. Oxalate and 2,5-dimethoxybenzoquinone were detected under all culture conditions, with higher production in high carbon/low nitrogen medium. Cultures grown in this medium also yielded the highest rate of hydroxylation of p-hydroxybenzoic acid, yielding protocatechuic acid, a product of hydroxyl radical attack.
机译：为了确定液体培养条件，表达棕色腐烂真菌Gloeophyllum trabeum的木材降解系统，在此条件下，对液体和固体底物培养物中的酶和代谢产物进行了表征。通过2-D凝胶电泳分析酶并进行测定。通过使用含有以下物质的液体培养基来改变生长条件：（1）低碳，低氮，（2）低碳，高氮，（3）高碳，低氮或（4）高碳，高氮。在这四个条件下表达的蛋白质阵列非常相似，并且检测到内吞1,4-β-葡聚糖酶（通过2-D凝胶检测）活性以及β-葡糖苷酶，木聚糖酶和NADH /醌氧化还原酶活性。在高碳/高氮培养基中观察到水解酶的最大表达，而最高的氧化还原酶活性在高碳低氮培养基中。在所有培养条件下均检测到草酸盐和2,5-二甲氧基苯醌，在高碳/低氮培养基中产量更高。在这种培养基中生长的培养物还产生了对羟基苯甲酸的最高羟基化速率，从而产生了原儿茶酸，即羟基自由基攻击的产物。

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