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首页> 外文期刊>Archives of microbiology >Identification of phototrophic sulfur bacteria through the analysis of ImwRNA band patterns
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Identification of phototrophic sulfur bacteria through the analysis of ImwRNA band patterns

机译：通过分析ImwRNA条带模式鉴定光养性硫细菌

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Several phototrophic sulfur bacteria were identified preliminarily through the analysis of the low-molecular-weight RNA fraction (lmwRNA) of bacterial cells. This fraction includes the ribosomal 5S RNA and several transfer RNAs. These molecules were separated by high-resolution electrophoresis in polyacrylamide gels, and the resulting band patterns were used as fingerprints for the identification of the organisms. We examined a large number of well-characterized reference strains together with a broad range of purple sulfur bacterial isolates from freshwater and marine environments. A cluster analysis was run using the similarity matrix calculated from the band patterns. Despite the shortcomings of the method, close relatives were clustered together yielding a number of groups consistent with the phylogenetic arrangement established through the analyses of a few available 16S rRNA gene sequences. Thus, the classification obtained gives further support to rearrangement of the group as the analyses of 16S rRNA gene sequences had previously suggested. We conclude that the analysis of lmwRNA band patterns is a rapid and simple tool for grouping and preliminarily identifying new isolates of phototrophic sulfur bacteria. [References: 33]
机译：通过分析细菌细胞的低分子量RNA分数（lmwRNA），初步鉴定出几种光养性硫细菌。该部分包括核糖体5S RNA和几个转移RNA。这些分子通过聚丙烯酰胺凝胶中的高分辨率电泳分离，所得的谱带图谱用作鉴定生物的指纹。我们检查了许多特征明确的参考菌株以及来自淡水和海洋环境的各种紫色硫细菌分离株。使用从条带模式计算的相似度矩阵进行聚类分析。尽管该方法有缺点，但将近亲聚类在一起，产生了许多与通过分析一些可用的16S rRNA基因序列而建立的系统发育安排一致的基团。因此，获得的分类为该组的重排提供了进一步的支持，因为先前已经提出了对16S rRNA基因序列的分析。我们得出结论，对lmwRNA条带模式的分析是一种快速而简单的工具，可用于分组和初步识别光养性硫细菌的新分离株。 [参考：33]

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