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Genes coding for respiratory complexes map on all three chromosomes of the Paracoccus denitrificans genome

机译:编码呼吸系统复合物的基因位于反硝化副球菌基因组的所有三个染色体上

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The genome of Paracoccus denitrificans (strain Pd1222) consists of three distinct DNA molecules when separated by standard pulsed-field gel electrophoresis with apparent molecular sizes of approximately 2, 1.1, and 0.64 Mb. When the separated chromosomes are digested by restriction enzymes and sizes of resulting fragments are summed up, the three chromosomes are composed of 1.83, 1.16, and 0.67 Mb. Since their migration behavior relative to size standards is largely independent of electrophoresis conditions, at least the two smaller chromosomes most likely represent linear molecules. The size analysis presented here allows an unequivocal distinction between groups of different strains of P. denitrificans and of Thiosphaera pantotropha, confirming an earlier cytochrome c analysis. When the genome was analyzed with different probes coding for respiratory enzymes, essential genes were found spread over all three chromosomes without any obvious clustering on any of the three forms. [References: 41]
机译:当通过标准脉冲场凝胶电泳分离时,反硝化副球菌(Pd1222株)的基因组由三个不同的DNA分子组成,其表观分子大小约为2、1.1和0.64 Mb。当分离的染色体被限制性内切酶消化并总结出片段大小时,这三个染色体分别由1.83、1.16和0.67 Mb组成。由于它们相对于尺寸标准的迁移行为在很大程度上与电泳条件无关,因此至少两个较小的染色体最有可能代表线性分子。此处提供的大小分析可以清楚地区分不同菌株的反硝化假单胞菌和泛硫酸化硫杆菌(Thiosphaera pantotropha),从而确认了较早的细胞色素c分析。当用不同的编码呼吸酶的探针对基因组进行分析时,发现必需基因分布在所有三个染色体上,而这三个形式中的任何一个都没有任何明显的聚集。 [参考:41]

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