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首页> 外文期刊>Archives of microbiology >Tetramethylammonium : coenzyme M methyltransferase system from Methanococcoides sp.
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Tetramethylammonium : coenzyme M methyltransferase system from Methanococcoides sp.

机译：四甲基铵：Methanococcocoides sp。的辅酶M甲基转移酶系统。

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A methanogen (strain NaT1) that belongs to the family of Methanosarcinaceae and that can grow on tetramethylammonium as the sole energy source has recently been isolated. We report here that cell extracts of the archaeon catalyze the formation of methyl-coenzyme M from coenzyme M and tetramethylammonium The activity was dependent on the presence of Ti(III) citrate and ATP, and was rapidly lost under oxic conditions. Anoxic chromatography on DEAE-Sepharose revealed that two fractions, fractions 3 and 4, were required for activity. A 50-kDa protein that together with fraction 3 catalyzed methyl-coenzyme M formation from tetramethylammonium and coenzyme M was purified from fraction 4. From fraction 3, a 22-kDa corrinoid protein and a 40-kDa protein exhibiting methylcobalamin:coenzyme M methyltransferase (MT2) activity were purified. The N-terminal amino acid sequences of these purified proteins were determined. The 40-kDa protein showed sequence similarity to MT2 isoenzymes from Methanosarcina barkeri. Cell extract of strain NaT1 grown on trimethylamine rather than on tetramethylammonium did not exhibit tetramethylammonium:coenzyme M methyltransferase activity. The strain was identified as belonging to the genus of Methanococcoides, its closest relative being Methanococcoides methylutens. [References: 41]
机译：最近已分离出一种属于甲烷菌科的产甲烷菌（NaT1菌株），它可以在四甲基铵上生长，作为唯一的能源。我们在这里报告，古细菌的细胞提取物催化由辅酶M和四甲基铵形成甲基辅酶M的活性取决于柠檬酸Ti（III）和ATP的存在，并且在有氧条件下迅速丧失。在DEAE-Sepharose上的缺氧色谱显示活性需要两个部分，部分3和4。从级分4中纯化了一个50 kDa的蛋白质，并与级分3催化了四甲基铵和辅酶M形成的甲基辅酶M的结合。从级分3中，一个22 kDa的类海藻蛋白和一个40 kDa的蛋白显示了甲基钴胺素：辅酶M甲基转移酶（纯化MT2）活性。确定了这些纯化蛋白的N-末端氨基酸序列。该40kDa蛋白显示与来自甲烷甲烷八叠球菌的MT2同工酶的序列相似性。在三甲胺而不是四甲基铵上生长的菌株NaT1的细胞提取物没有表现出四甲基铵：辅酶M甲基转移酶活性。该菌株被鉴定为属于甲烷球菌属，其最接近的亲属是甲基甲烷球菌。 [参考：41]

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