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首页> 外文期刊>Archives of microbiology >Growth, natural relationships, cellular fatty acids and metabolic adaptation of sulfate-reducing bacteria that utilize long-chain alkanes under anoxic conditions
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Growth, natural relationships, cellular fatty acids and metabolic adaptation of sulfate-reducing bacteria that utilize long-chain alkanes under anoxic conditions

机译：在缺氧条件下利用长链烷烃的硫酸盐还原细菌的生长，自然关系，细胞脂肪酸和代谢适应

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Natural relationships, improvement of anaerobic growth on hydrocarbons, and properties that may provide clues to an understanding of oxygen-independent alkane metabolism were studied with two mesophilic sulfate-reducing bacteria, strains Hxd3 and Pnd3. Strain Hxd3 had been formerly isolated from an oil tank; strain Pnd3 was isolated from marine sediment. Strains Hxd3 and Pnd3 grew under strictly anoxic conditions on n-alkanes in the range of C-12-C-20 and C-14-C-17, respectively, reducing sulfate to sulfide. Both strains shared 90% 16 S rRNA sequence similarity and clustered with classified species of completely oxidizing, sulfate-reducing bac bacteria within the delta-subclass of Proteobacteria. Anaerobic growth on alkanes was stimulated by alpha-cyclodextrin, which served as a non-degradable carrier for the hydrophobic substrate. Cells of strain Hxd3 grown on hydrocarbons and alpha-cyclodextrin were used to study the composition of cellular fatty acids and in vivo activities. When strain Hxd3 was grown on hexadecane (C16H34), cellular fatty acids with C-odd chains were dominant. Vice versa, cultures grown on heptadecane (C17H36) contained mainly fatty acids with C-even chains. In contrast, during growth on 1-alkenes or fatty acids, a C-even substrate yielded C-even fatty acids, and a C-odd substrate yielded C-odd fatty acids. These results suggest that anaerobic degradation of alkanes by strain Hxd3 does not occur via a desaturation to the corresponding 1-alkenes, a hypothetical reaction formerly discussed in the literature. Rather an alteration of the carbon chain by a C-odd carbon unit is likely to occur during activation; one hypothetical reaction is a terminal addition of a C-1, unit. In contrast, fatty acid analyses of strain Pnd3 after growth on alkanes did not indicate an alteration of the carbon chain by a C-odd carbon unit, suggesting that the initial reaction differed from that in strain Hxd3. When hexadecane-grown cells of strain Hxd3 were resuspended in medium with 1-hexadecene, an adaptation period of 2 days was observed. Also this result is not in favor of an anaerobic alkane degradation via the corresponding l-alkene. [References: 55]
机译：用两种嗜温性硫酸盐还原细菌（菌株Hxd3和Pnd3）研究了自然关系，碳氢化合物厌氧生长的改善以及可能为了解不依赖氧的烷烃代谢提供线索的性质。 Hxd3菌株以前是从一个油箱中分离出来的。从海洋沉积物中分离出菌株Pnd3。菌株Hxd3和Pnd3在严格缺氧条件下分别在C-12-C-20和C-14-C-17范围内的正构烷烃上生长，从而将硫酸盐还原为硫化物。两种菌株都具有90％的16 S rRNA序列相似性，并与变形细菌三角洲亚类中完全氧化的，可还原硫酸盐还原的bac细菌分类。 α-环糊精刺激烷烃上的厌氧生长，α-环糊精充当疏水性底物的不可降解载体。使用在碳氢化合物和α-环糊精上生长的菌株Hxd3的细胞研究细胞脂肪酸的组成和体内活性。当菌株Hxd3在十六烷（C16H34）上生长时，具有C奇数链的细胞脂肪酸占主导地位。反之亦然，在庚烷（C17H36）上生长的培养物主要含有带有C-even链的脂肪酸。相反，在1-烯烃或脂肪酸上生长期间，C-偶数底物产生C-偶数脂肪酸，而C-奇数底物产生C-奇数脂肪酸。这些结果表明，Hxd3菌株不会通过对相应的1-烯烃进行去饱和而发生厌氧降解，这是以前在文献中讨论过的一种假设反应。相反，在活化过程中很可能会发生C奇数碳单元对碳链的改变。一个假设的反应是C-1单元的末端加成。相反，在烷烃上生长后对菌株Pnd3进行的脂肪酸分析未显示碳数为C的碳单元对碳链的改变，这表明初始反应与菌株Hxd3不同。当十六烷生长的菌株Hxd3的细胞重悬于含有1-十六烯的培养基中时，观察到的适应期为2天。同样，该结果也不利于通过相应的1-烯烃的厌氧烷烃降解。 [参考：55]

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