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首页> 外文期刊>Archives of microbiology >Evaluation of pink-pigmented facultative methylotrophic bacteria for phosphate solubilization
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Evaluation of pink-pigmented facultative methylotrophic bacteria for phosphate solubilization

机译：粉色色素兼性甲基营养细菌对磷酸盐增溶的评价

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Thirteen pink-pigmented facultative methylotrophic (PPFM) strains isolated from Adyar and Cooum rivers in Chennai and forest soil samples in Tamil Nadu, India, along with Methylobacterium extorquens, M. organophilum, M. gregans, and M. komagatae were screened for phosphate solubilization in plates. P-solubilization index of the PPFMs grown on NBRIP-BPB plates for 7 days ranged from 1.1 to 2.7. The growth of PPFMs in tricalcium phosphate amended media was found directly proportional to the glucose concentration. Higher phosphate solubilization was observed in four strains MSF 32 (415 mg l-l), MDW 80 (301 mg l-l), M. komagatae (279 mg l-l), and MSF 34 (202 mg l-l), after 7 days of incubation. A drop in the media pH from 6.6 to 3.4 was associated with an increase in titratable acidity. Acid phosphatase activity was more pronounced in the culture filtrate than alkaline phosphatase activity. Adherence of phosphate to densely grown bacterial surface was observed under scanning electron microscope after 7-day-old cultures. Biochemical characterization and screening for methanol dehydrogenase gene (mxaF) confirmed the strains as methylotrophs. The mxaF gene sequence from MSF 32 clustered towards M. lusitanum sp. with 99% similarity. This study forms the first detailed report on phosphate solubilization by the PPFMs.
机译：从印度钦奈的Adyar和Cooum河以及印度泰米尔纳德邦的森林土壤样品中分离出的13种粉红色色素兼性甲基营养型（PPFM）菌株与过氧化甲基杆菌，M.organophilum，M。gregans和komagatae一起被筛选了磷酸盐增溶作用在盘子里。在NBRIP-BPB平板上生长7天的PPFM的P增溶指数范围为1.1至2.7。发现PPFM在磷酸三钙改良培养基中的生长与葡萄糖浓度成正比。温育7天后，在四种菌株MSF 32（415 mg 1-1），MDW 80（301 mg 1-1），komagatae（279 mg 1-1）和MSF 34（202 mg 1-1）中观察到更高的磷酸盐增溶。培养基pH值从6.6降至3​​.4与可滴定酸度增加有关。培养滤液中的酸性磷酸酶活性比碱性磷酸酶活性更明显。培养7天后，在扫描电子显微镜下观察到磷酸盐对致密生长的细菌表面的粘附。生化特征和甲醇脱氢酶基因（mxaF）的筛选证实了该菌株为甲基营养菌。来自MSF 32的mxaF基因序列聚簇至lusitanum sp。具有99％的相似性。这项研究形成了有关PPFM溶解磷酸盐的第一份详细报告。

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