• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Archives of microbiology >Characterization of the group 1 and group 2 sigma factors of the green sulfur bacterium Chlorobium tepidum and the green non-sulfur bacterium Chloroflexus aurantiacus
【24h】

Characterization of the group 1 and group 2 sigma factors of the green sulfur bacterium Chlorobium tepidum and the green non-sulfur bacterium Chloroflexus aurantiacus

机译：绿色硫细菌绿脓杆菌和绿色非硫细菌黄绿弯曲杆菌第1组和第2组sigma因子的表征

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The group I and group 2 sigma(70)-type sigma factors of the green sulfur bacterium Chlorobium tepidum and of the green nonsulfur bacterium Chloroflexus aurantiacus were cloned and characterized. Cb. tepidum was found to contain one sigma 70-type sigma factor; the expression of the gene was analyzed by Northern blot hybridization and primer-extension mapping. Cf. aurantiacus has genes encoding four sigma factors of groups 1 and 2. The expression of these genes was examined in cells grown aerobically and anaerobically. The sigC gene was expressed at approximately equal levels under both conditions, resulting in its designation as the group 1 sigma factor of this organism. The only other detectable transcripts arose from the sigB gene, which was expressed at higher levels during aerobic growth. A phylogenetic tree was obtained using the group 1 sigma factors of Cb. tepidum, Cf. aurantiacus, and diverse eubacteria as the molecular marker. The resulting phylogenetic tree shows that Cb. tepidum and Cf. aurantiacus are related to each other and to the cyanobacteria. The relationship of the group 2 sigma factors of Cf. aurantiacus and the cyanobacteria was more specifically examined phylogenetically. The group 2 sigma factors of Cf. aurantiacus probably arose by gene duplication events after the split of the green nonsulfur bacteria from other photosynthetic eubacteria. [References: 63]
机译：克隆并鉴定了绿色硫细菌绿脓杆菌和绿色非硫细菌绿藻弯曲杆菌的I组和2组σ（70）型σ因子。 b发现温热菌含有一个sigma 70型sigma因子;通过Northern印迹杂交和引物延伸图谱分析该基因的表达。 cf. aurantiacus具有编码第1组和第2组四个sigma因子的基因。在需氧和厌氧的细胞中检查了这些基因的表达。 sigC基因在两种条件下均以近似相等的水平表达，因此被指定为该生物的1组sigma因子。唯一其他可检测到的转录物来自sigB基因，在有氧生长过程中以更高的水平表达。使用Cb组的1σ因子获得了系统发育树。特皮德姆，CF。紫罗兰色和多种真细菌作为分子标记。所得的系统发育树显示Cb。温和和CF。奥兰蒂亚克斯彼此相关并且与蓝细菌相关。 Cf 2组西格玛因子的关系。更详细地检查了金黄色葡萄球菌和蓝细菌。 Cf的第2组sigma因子。绿色的无硫细菌从其他光合真细菌中分裂出来后，基因复制事件引起了金黄色葡萄球菌的生长。 [参考：63]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号