首页> 外文期刊>Archives of microbiology >Isolation and characterization of Desulfovibrio senezii sp. nov., a halotolerant sulfate reducer from a solar saltern and phylogenetic confirmation of Desulfovibrio fructosovorans as a new species
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Isolation and characterization of Desulfovibrio senezii sp. nov., a halotolerant sulfate reducer from a solar saltern and phylogenetic confirmation of Desulfovibrio fructosovorans as a new species

机译:Desulfovibrio senezii sp。的分离和鉴定。十一月,从一种盐法制盐的硫酸盐卤化还原剂,并在系统发育学中证实了果糖脱硫新种

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摘要

A new halotolerant Desulfovibrio, strain CVLT (T = type strain), was isolated from a solar saltern in California. The curved, gram-negative, nonsporeforming cells (0.3 x 1.0-1.3 mu m) occurred singly, in pairs, or in chains, were motile by a single polar flagellum and tolerated up to 12.5% NaCl. Strain CVLT had a generation time of 60 min when grown in lactate-yeast extract medium under optimal conditions (37 degrees C, pH 7.6, 2.5% NaCl). It used lactate, pyruvate, cysteine, or H-2/CO2 + acetate as electron donors, and sulfate, sulfite, thiosulfate, or fumarate as electron accepters. Elemental sulfur, nitrate, or oxygen were not used. Sulfite and thiosulfate were disproportionated to sulfate and sulfide. The G+C content of the DNA was 62 mol%. Phylogenetic analysis revealed that Desulfovibrio fructosovorans was the nearest relative. Strain CVLT is clearly different from other Desulfovibrio species, and is designated Desulfovibrio senezii sp. nov. (DSM 8436). [References: 30]
机译:从加利福尼亚的一个太阳能盐分离器中分离出一种新的耐盐脱硫弧菌,菌株CVLT(T =型菌株)。弯曲的,革兰氏阴性的非孢子形成细胞(0.3 x 1.0-1.3微米)单独,成对或成链出现,由单极鞭毛游动并能耐受高达12.5%的NaCl。在最佳条件下(37摄氏度,pH 7.6、2.5%NaCl)在乳酸酵母发酵培养基中培养,CVLT菌株的生成时间为60分钟。它使用乳酸,丙酮酸,半胱氨酸或H-2 / CO2 +乙酸盐作为电子供体,并使用硫酸盐,亚硫酸盐,硫代硫酸盐或富马酸盐作为电子受体。不使用元素硫,硝酸盐或氧气。亚硫酸盐和硫代硫酸盐歧化为硫酸盐和硫化物。 DNA的G + C含量为62mol%。系统发育分析表明,Desulfovibrio fructosovorans是最近的亲戚。菌株CVLT明显不同于其他Desulfovibrio菌种,被命名为Desulfovibrio senezii sp。十一月(DSM 8436)。 [参考:30]

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