首页> 外文期刊>Archives of microbiology >FORMATION OF ACTIVE NITRITE REDUCTASE (CYTOCHROME CD(1)) IN THE STRAIN PARACOCCUS DENITRIFICANS HUUG25
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FORMATION OF ACTIVE NITRITE REDUCTASE (CYTOCHROME CD(1)) IN THE STRAIN PARACOCCUS DENITRIFICANS HUUG25

机译:菌株Paracoccus denitrificans的活性亚硝酸还原酶(细胞色素CD(1))的形成HUUG25

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Strain HUUG25 of Paracoccus denitrificans has been frequently thought to be devoid of all c-type cytochromes. We show here by means of enzymological and immunological techniques that the mutant synthesizes active nitrite reductase (cytochrome cd(1)) upon prolonged exposure to microoxic conditions. The synthesis occurred faster in the presence of exogenous hemin, The time pattern of 5-aminolevulinate synthase activity was also altered by the mutation. These findings suggest a defective regulation of heme supply to the site of nitrite reductase assembly in the periplasm. [References: 30]
机译:人们经常认为反硝化副球菌的菌株HUUG25不含所有c型细胞色素。我们在这里通过酶学和免疫学技术表明,该突变体在长时间暴露于微氧条件下会合成活性亚硝酸盐还原酶(细胞色素cd(1))。在存在外源血红素的情况下,合成发生得更快。5-氨基乙酰丙酸合酶活性的时间模式也因突变而改变。这些发现表明血红素对周质中亚硝酸还原酶组装位点的血红素供应的调节有缺陷。 [参考:30]

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