首页> 外文期刊>Archives of microbiology >THE EXPRESSION OF REDOX PROTEINS OF DENITRIFICATION IN THIOSPHAERA PANTOTROPHA GROWN WITH OXYGEN, NITRATE, AND NITROUS OXIDE AS ELECTRON ACCEPTORS
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THE EXPRESSION OF REDOX PROTEINS OF DENITRIFICATION IN THIOSPHAERA PANTOTROPHA GROWN WITH OXYGEN, NITRATE, AND NITROUS OXIDE AS ELECTRON ACCEPTORS

机译:以氧,硝酸盐和一氧化二氮为电子受体的泛营养化硫氧化还原蛋白的表达

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The redox proteins and enzymes involved in denitrification in Thiosphaera pantotropha exhibited a differential expression in response to oxygen. Pseudoazurin was completely repressed during batch or continuous culture under oxic conditions. Cytochrome ed, nitrite reductase was also heavily repressed after aerobic growth. Nitrite, nitric oxide, and nitrous oxide reductase activities were detected in intact cells under some conditions of aerobic growth, indicating that aerobic denitrification might occur in some circumstances. However, the rates of denitrification were much lower after aerobic growth than after anaerobic growth. Growth with nitrous oxide as sole electron acceptor mimicked aerobic growth in some respects, implying that expression of parts of the denitrification apparatus might be controlled by the redox state of a component of the electron transport chain rather than by oxygen itself. Nevertheless, the regulation of expression of nitrous oxide reductase was linked to the oxygen concentration. [References: 30]
机译:泛硫硫杆菌中反硝化作用的氧化还原蛋白和酶表现出对氧气的差异表达。在有氧条件下分批或连续培养期间,伪天青素被完全抑制。有氧生长后,细胞色素,亚硝酸盐还原酶也被严重抑制。在某些有氧生长条件下,在完整细胞中检测到亚硝酸盐,一氧化氮和一氧化二氮还原酶的活性,这表明在某些情况下可能发生有氧反硝化作用。但是,有氧生长后的反硝化率要比厌氧生长后的低。一氧化二氮作为唯一的电子受体的生长在某些方面模仿了有氧生长,这意味着反硝化装置各部分的表达可能受电子传输链成分的氧化还原状态控制,而不是受氧本身控制。然而,一氧化二氮还原酶表达的调节与氧浓度有关。 [参考:30]

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