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首页> 外文期刊>Archives of microbiology >Arsenate detoxification in a Pseudomonad hypertolerant to arsenic
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Arsenate detoxification in a Pseudomonad hypertolerant to arsenic

机译：对砷具有高度耐受性的假单胞菌中的砷酸盐解毒

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Pseudomonas sp. strain As-1, obtained from an electroplating industrial effluent, was capable of growing aerobically in growth medium supplemented with up to 65 mM arsenate (As (V)), significantly higher concentrations than those tolerated by other reference arsenic resistant bacteria. The majority of the arsenic was detected in culture supernatants as arsenite (As (III)) and X-ray absorbance spectroscopy suggested that 30% of this cell-bound arsenic was As (V), 65% As (III) and 5% of arsenic was associated with sulphur. PCR analysis using primers designed against arsenic resistance genes of other Gram-negative bacteria confirmed the presence of an arsenic resistance operon comprising of three genes, arsR, arsB and arsC in order of predicted transcription, and consistent with a role in intracellular reduction of As (V) and efflux of As (III). In addition to this classical arsenic resistance mechanism, other biochemical responses to arsenic were implicated. Novel arsenic-binding proteins were purified from cellular fractions, while proteomic analysis of arsenic-induced cultures identified the upregulation of additional proteins not normally associated with the metabolism of arsenic. Cross-talk with a network of proteins involved in phosphate metabolism was suggested by these studies, consistent with the similarity between the phosphate and arsenate anions.
机译：假单胞菌从电镀工业废水中获得的As-1菌株能够在添加至多65 mM砷酸盐（As（V））的生长培养基中有氧生长，其浓度明显高于其他抗砷参考细菌所耐受的浓度。在培养上清液中检测到的大部分砷为亚砷酸盐（As（III）），X射线吸收光谱表明，细胞结合的砷中有30％是As（V），65％As（III）和5％砷与硫有关。使用针对其他革兰氏阴性细菌的抗砷基因设计的引物进行PCR分析，证实存在抗砷操纵子，该操纵子由三个基因arsR，arsB和arsC组成，按预测的转录顺序排列，并与As的细胞内还原作用一致（ V）和As（III）的外排。除了这种经典的抗砷机理外，还涉及对砷的其他生化反应。从细胞级分中纯化了新型的砷结合蛋白，同时对砷诱导的培养物进行蛋白质组学分析，确定了通常与砷代谢无关的其他蛋白质的上调。这些研究表明，与磷酸盐代谢中涉及的蛋白质网络发生串扰，这与磷酸盐和砷酸根阴离子之间的相似性相一致。

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