• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Archives of microbiology >Cell aggregation of Pseudomonas aeruginosa strain PAO1 as an energy-dependent stress response during growth with sodium dodecyl sulfate
【24h】

Cell aggregation of Pseudomonas aeruginosa strain PAO1 as an energy-dependent stress response during growth with sodium dodecyl sulfate

机译：十二烷基硫酸钠生长过程中铜绿假单胞菌PAO1的细胞聚集作为能量依赖的应激反应

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Pseudomonas aeruginosa strain PAO1 grew with the detergent sodium dodecyl sulfate (SDS). The growth started with the formation of macroscopic cell aggregates which consisted of respiring cells embedded in an extracellular matrix composed of acidic polysaccharides and DNA. Damaged and uncultivable cells accumulated in these aggregates compared to those cells that remained suspended. We investigated the response of suspended cells to SDS under different conditions. At high energy supply, the cells responded with a decrease in optical density and in viable counts, release of protein and DNA, and formation of macroscopic aggregates. This response was not observed if the energy supply was reduced by inhibiting respiration with KCN, or if cells not induced for SDS degradation were exposed to SDS. Exposure to SDS caused cell lysis without aggregation if cells were completely deprived of energy, either by applying anoxic conditions, by addition of CCCP, or by addition of KCN to a mutant defective in cyanide-insensitive respiration. Aggregated cells showed a more than 100-fold higher survival rate after exposure to SDS plus CCCP than suspended cells. Our results demonstrate that cell aggregation is an energy-dependent response of P. aeruginosa to detergent stress which might serve as a survival strategy during growth with SDS.
机译：铜绿假单胞菌菌株PAO1与去污剂十二烷基硫酸钠（SDS）一起生长。生长始于宏观细胞聚集体的形成，其由嵌入酸性多糖和DNA组成的细胞外基质中的呼吸细胞组成。与那些保持悬浮状态的细胞相比，受损和无法培养的细胞在这些聚集物中积累。我们调查了悬浮细胞对SDS在不同条件下的反应。在高能量供应下，细胞的反应是光密度和活计数的降低，蛋白质和DNA的释放以及宏观聚集体的形成。如果通过抑制KCN呼吸来减少能量供应，或者如果未诱导SDS降解的细胞暴露于SDS，则不会观察到此响应。如果通过施加缺氧条件，通过添加CCCP或通过将KCN添加到对氰化物不敏感的呼吸缺陷的突变体中，完全暴露了能量，暴露于SDS会使细胞裂解而不会聚集。暴露于SDS加CCCP后，聚集细胞的存活率比悬浮细胞高100倍以上。我们的结果表明，细胞聚集是铜绿假单胞菌对去污剂胁迫的能量依赖性反应，这可能是SDS生长期间的生存策略。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号