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首页> 外文期刊>Archives of microbiology >Hydrogen uptake in Nostoc sp. strain PCC 73102. Cloning and characterization of a hupSL homologue
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Hydrogen uptake in Nostoc sp. strain PCC 73102. Cloning and characterization of a hupSL homologue

机译：Nostoc sp。中的氢吸收。株PCC73102。hupSL同源物的克隆和鉴定

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Structural genes encoding an uptake hydrogenase of Nostoc sp. strain PCC 73102 were isolated. From partial libraries of genomic DNA, two clones (pNfo01 and pNfo02) were selected and sequenced, revealing the complete sequence of both a hupS (960 bases) and a hupL (1,593 bases) homologue in Nostoc sp. strain PCC 73102. A comparison between the deduced amino acid sequences of HupS and HupL of Nostoc sp. strain PCC 73102 and Anabaena sp. strain PCC 7120 showed that the HupS proteins are 89% identical and the HupL proteins are 91% identical. However, the noncoding region between the genes in Nostoc sp. strain PCC 73102 (192 bases) is longer than that of Anabaena sp. strain PCC 7120 and of many other microorganism. Southern hybridizations using DNA from both N-2-fixing and non-N-2-fixing cells of Nostoc sp. strain PCC 73102 and different probes from within hupL clearly demonstrated that, in contrast to Anabaena sp. strain PCC 7120, there is no rearrangement within hupL Of Nostoc Sp. Strain PCC 73102. Indeed, 6 nucleotides out of 16 within the potential recombination site are different from those of Anabaena sp. strain PCC 7120. Furthermore, we have recently published evidence demonstrating the absence of the bidirectional/reversible hydrogenase in Nostoc sp. strain PCC 73102. The present knowledge, in combination with the unique characteristics, makes Nostoc sp, strain PCC 73102 an interesting candidate for the study of deletion mutants lacking the uptake-type enzyme. [References: 32]
机译：编码Nostoc sp。摄取氢化酶的结构基因。分离出PCC 73102菌株。从基因组DNA的部分文库中，选择了两个克隆（pNfo01和pNfo02）并进行了测序，揭示了Nostoc sp。中hupS（960个碱基）和hupL（1,593个碱基）同源物的完整序列。 Nostoc sp。的HupS和HupL的推导氨基酸序列之间的比较。 PCC 73102菌株和Anabaena sp。 PCC 7120菌株显示HupS蛋白具有89％的同一性，而HupL蛋白具有91％的同一性。然而，在Nostoc sp。基因中的非编码区。 PCC 73102菌株（192个碱基）比Anabaena sp。更长。株PCC 7120和许多其他微生物。使用来自Nostoc sp。的N-2固定和非N-2固定细胞的DNA进行Southern杂交。 hupL内的PCC 73102菌株和不同探针清楚地表明，与Anabaena sp。菌株PCC 7120，在Nostoc Sp的hupL中没有重排。菌株PCC73102。实际上，潜在重组位点内16个核苷酸中的6个核苷酸与鱼腥藻属（Anabaena sp。）的核苷酸不同。此外，我们最近发表了证据，证明Nostoc sp。中不存在双向/可逆氢化酶。菌株PCC 73102的本知识与独特的特性相结合，使Nostoc sp，PCC 73102菌株成为研究缺乏摄取型酶的缺失突变体的有趣候选者。 [参考：32]

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