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首页> 外文期刊>Archives of microbiology >Characterization of a mutant strain of Rhodovulum sulfidophilum lacking the pufA and pufB genes encoding the polypeptides for the light-harvesting complex 1 (B 870)
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Characterization of a mutant strain of Rhodovulum sulfidophilum lacking the pufA and pufB genes encoding the polypeptides for the light-harvesting complex 1 (B 870)

机译：缺少编码光捕获复合物1多肽的pufA和pufB基因的硫化硫红球菌突变株的表征（B 870）

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摘要

Contradictory results on the effectiveness of energy transfer from the light harvesting complex 2 (LH2) directly to the reaction center (RC) in mutant strains lacking the core light-harvesting complex 1 (LH1) have been obtained with cells of Rhodobacter capsulatus and Rhodobacter sphaeroides. A LH1(-) mutant of Rhodovulum sulfidophilum, named rsLRI, was constructed by deletion of the pufBA genes, resulting in a kanamycin resistant photosynthetically positive clone. To restore the wild type phenotype, a complemented strain C2 was constructed by inserting in trans a DNA segment containing the pufBA genes. Light-induced FTIR difference spectra indicate that the RC in the rsLRI mutant and in the C2 complemented strains are functionally and structurally identical with those in the wild type strain, demonstrating that the assembly and the function of the RC is not impaired by the LH1 deletion. The photosynthetic growth rate of the rsLRI strain increased with decreasing light intensity. At 50 W m(-2) no photosynthetic growth was observed. These results indicate that the light energy harvested by the LH2 complex was not or inefficiently transferred to the RC; thus most of the energy necessary for photosynthetic growth is in the LH1(-) strain directly absorbed by the RC. It is supposed that in the mutant strain, RC and LH2 cannot interact in an efficient way.
机译：在荚膜红球菌和球形红球菌的细胞中，在缺乏核心光捕获复合物1（LH1）的突变菌株中，从光捕获复合物2（LH2）直接向反应中心（RC）转移能量的有效性产生了矛盾的结果。 。通过缺失pufBA基因，构建了一个名为rsLRI的嗜硫球藻的LH1（-）突变体，从而产生了抗卡那霉素的光合阳性克隆。为了恢复野生型表型，通过反式插入含有pufBA基因的DNA片段来构建互补菌株C2。光诱导的FTIR差异光谱表明rsLRI突变体和C2互补菌株中的RC在功能和结构上与野生型菌株中的RC相同，表明RC的组装和功能不受LH1缺失的影响。 。 rsLRI菌株的光合生长速率随光强度的降低而增加。在50 W m（-2）没有观察到光合作用的增长。这些结果表明，LH2络合物收集的光能没有或没有有效地转移到RC。因此，光合作用生长所需的大部分能量都在RC直接吸收的LH1（-）菌株中。据推测，在突变菌株中，RC和LH2不能有效地相互作用。

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