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首页> 外文期刊>Archives of microbiology >Investigation of the functional properties and regulation of three glutamine synthetase-like genes in Streptomyces coelicolor A3(2)
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Investigation of the functional properties and regulation of three glutamine synthetase-like genes in Streptomyces coelicolor A3(2)

机译：链霉菌A3（2）中三个谷氨酰胺合成酶样基因的功能特性和调控研究

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Streptomyces coelicolor A3(2) has three additional glnA-type genes besides the glutamine synthetase genes glnA (encoding GSI) and glnII (encoding GSII). The aim of this work was to characterize their functional properties and regulation. Sequence analyses revealed that GlnA2, GlnA3, and GlnA4 are dissimilar to S. coelicolor GSI and lack highly conserved amino acid residues involved in catalysis. In heterologous expression experiments, glnA2, glnA3, and glnA4, in contrast to glnA and glnII, were not capable of complementing the L-glutamine auxotrophy of an Escherichia coli glnA mutant. The lack of a conserved sequence motif reflecting adenylylation control of enzyme activity suggests that GlnA2, GlnA3, and GlnA4 are not regulated via adenylyltransferase-mediated modification. In DNA-binding assays, the OmpR-like regulator of nitrogen metabolism GlnRII, which interacts with the glnA and glnII promoters, did not bind to the upstream regions of glnA2, glnA3, and glnA4. These findings support the conclusion that glnA2, glnA3, and glnA4 are not directly involved in L-glutamine synthesis and nitrogen assimilation and are not subject to nitrogen control in S. coelicolor. The glnA3 gene product is similar to FluG, which is required for asexual sporulation in Aspergillus nidulans. However, inactivation of glnA3 does not block morphological differentiation in S. coelicolor.
机译：除谷氨酰胺合成酶基因glnA（编码GSI）和glnII（编码GSII）外，天蓝色链霉菌A3（2）还具有另外三个glnA型基因。这项工作的目的是表征其功能特性和调节。序列分析表明，GlnA2，GlnA3和GlnA4与天蓝色链霉菌GSI不同，并且缺少参与催化的高度保守的氨基酸残基。在异源表达实验中，与glnA和glnII相比，glnA2，glnA3和glnA4无法补充大肠杆菌glnA突变体的L-谷氨酰胺营养缺陷型。缺乏反映酶活性的腺苷酸化控制的保守序列基序的缺乏表明，GlnA2，GlnA3和GlnA4不受腺苷酸转移酶介导的修饰的调控。在DNA结合测定中，与glnA和glnII启动子相互作用的氮代谢GlnRII的OmpR样调节剂不与glnA2，glnA3和glnA4的上游区域结合。这些发现支持以下结论：glnA2，glnA3和glnA4不直接参与L-谷氨酰胺合成和氮同化，并且不受制于天蓝色链霉菌中的氮。 glnA3基因产物类似于FluG，这是构巢曲霉无性孢子形成所必需的。但是，glnA3的失活不会阻止天蓝色链霉菌的形态分化。

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