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首页> 外文期刊>Archives of microbiology >A high-conductance cation channel from the inner membrane of the free-living soil bacteria Rhizobium etli.
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A high-conductance cation channel from the inner membrane of the free-living soil bacteria Rhizobium etli.

机译：来自自由生活土壤细菌根瘤菌内膜的高电导阳离子通道。

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摘要

In this communication we reported the study of a cation channel present in the cytoplasmic membrane of the nitrogen fixing bacterium Rhizobium etli. Inner-membrane (IM) vesicles were purified and fused into planar lipid bilayers (PLBs), under voltage clamp conditions. We have found that fusion of IM-enriched vesicle fractions with these model membranes leads, mainly (>30% of 46 experiments), to the reconstitution of high-conductance channels. Following this strategy, the activity of a channel with main open conductance of 198 pS, in symmetrical 100 mM KCl, was recorded. The single-channel conductance increase to 653 pS in the presence of a 5:1 (cis to trans) gradient of KCl. The channel exhibits voltage dependency and a weak selectivity for cations showing a permeability ratios of P (Rb)/P (K) = 0.96, P (Na)/P (K) = 0.07, and a conductance ratio of gamma(Rb)/gamma(K) = 1.1. The channel here characterized represents a previously undescribed Rhizobium channel although its precise role in rhizobial physiology remains yet to be determined.
机译：在本次交流中，我们报道了对固氮细菌根瘤菌（Rhizobium etli）细胞质膜中存在的阳离子通道的研究。内膜（IM）囊泡被纯化，并在电压钳制条件下融合到平面脂质双层（PLB）中。我们已经发现，富含IM的囊泡部分与这些模型膜的融合主要导致高传导通道的重建（> 46个实验的30％）。按照该策略，记录了在对称的100 mM KCl中主要开放电导为198 pS的通道的活动。在存在5：1（顺式至反式）KCl梯度的情况下，单通道电导增加至653 pS。该通道表现出电压依赖性和对阳离子的弱选择性，其渗透率比为P（Rb）/ P（K）= 0.96，P（Na）/ P（K）= 0.07，电导比为gamma（Rb）/ γ（K）= 1.1。这里表征的通道代表以前未描述的根瘤菌通道，尽管其在根瘤菌生理学中的确切作用仍有待确定。

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