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SUCCINATE DECARBOXYLATION BY PROPIONIGENIUM MARIS SP NOV, A NEW ANAEROBIC BACTERIUM FROM AN ESTUARINE SEDIMENT

机译:食盐沉积物中一种新型厌氧细菌丙酸丙啶菌新戊酸酯的脱羧作用

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Enrichments on succinate plus yeast extract under anoxic conditions from intertidal mud-flat sediments yielded cultures dominated by oval to round-ended rod-shaped cells. Strain 10succl, obtained in pure culture, was characterized in detail. The non-motile cells possessed a gram-negative cell wall and did not form spores. Carbohydrates were fermented to formate, acetate, ethanol, and lactate. Succinate was decarboxylated to propionate. Other organic and amino acids were variously fermented to formate, acetate, propionate, and butyrate. Sulfur, sulfate, thiosulfate, and nitrate were not used as electron accepters, Growth required the presence of yeast extract and at least 5 g/l NaCl, and was possible only in the absence of oxygen. No cytochromes were detected. The DNA base ratio was 40 mol% G+C. Phylogenetically, strain 10succl is closely related to Propionigenium modestum, as revealed by 16S rDNA analysis, but is physiologically distinct. Accordingly, strain 10succl (DSM 9537) is described as the type strain of a new species of the genus Propionigenium, P. maris sp. nov. [References: 34]
机译:在缺氧条件下,从潮间带泥滩沉积物中富集琥珀酸盐和酵母提取物,产生的培养物以椭圆形到圆端的杆状细胞为主。对纯培养物中获得的10succl菌株进行了详细表征。非运动细胞具有革兰氏阴性细胞壁并且不形成孢子。将碳水化合物发酵成甲酸盐,乙酸盐,乙醇和乳酸盐。琥珀酸酯脱羧成丙酸酯。将其他有机和氨基酸发酵成甲酸酯,乙酸酯,丙酸酯和丁酸酯。硫,硫酸盐,硫代硫酸盐和硝酸盐未用作电子受体,生长需要存在酵母提取物和至少5 g / l NaCl,并且仅在没有氧气的情况下才可能生长。未检测到细胞色素。 DNA碱基比率是40mol%G + C。在系统发育上,如16S rDNA分析所揭示的,菌株10succl与模式圈霉菌密切相关,但在生理上却截然不同。因此,菌株10succl(DSM 9537)被描述为Propionigenium属的新种P. maris sp。的类型菌株。十一月[参考:34]

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