TETRAMERIC STRUCTURE AND CELLULAR LOCATION OF CATECHOL 2,3-DIOXYGENASE

Winkler J.; Dwyer DF.; Rohde M.; Eltis LD.

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TETRAMERIC STRUCTURE AND CELLULAR LOCATION OF CATECHOL 2,3-DIOXYGENASE

机译：儿茶酚2,3-二氧合酶的四聚体结构和细胞定位

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Catechol 2,3-dioxygenase from the meta-cleavage pathway encoded on the TOL plasmid of Pseudomonas putida (pWWO) was investigated by electron microscopy. Negatively stained samples of the purified catechol 2,3-dioxygenase revealed that the enzyme consists of four subunits arranged in a tetrahedral conformation. Monoclonal antibodies raised against catechol 2,3-dioxygenase showed highly specific reactions and were used to localize the enzyme in Escherichia coli (pAW31) and P. putida (pWWO), using the protein A-gold technique carried out as a post-embedding immunoelectron microscopy procedure. Our in situ labeling studies revealed a cytoplasmic location of the catechol 2,3-dioxygenase in both cell types. [References: 25]

机译：通过电子显微镜研究了恶臭假单胞菌（pWWO）的TOL质粒上编码的间位裂解途径中的邻苯二酚2,3-二加氧酶。纯化的邻苯二酚2,3-二加氧酶的负染色样品显示，该酶由排列成四面体构象的四个亚基组成。针对儿茶酚2,3-双加氧酶的单克隆抗体表现出高度特异性的反应，并使用蛋白A-金技术作为包埋后免疫电子技术，将该酶定位于大肠杆菌（pAW31）和恶臭假单胞菌（pWWO）中。显微镜检查程序。我们的原位标记研究揭示了两种细胞类型中邻苯二酚2,3-二加氧酶的胞质位置。 [参考：25]

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《Archives of microbiology》 |1995年第1期|共5页
作者
Winkler J.; Dwyer DF.; Rohde M.; Eltis LD.;
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正文语种 eng
中图分类医学微生物学（病原细菌学、病原微生物学）;植物学;
关键词
Catechol 2; 3-dioxygenase; Pseudomonas putida; Tol plasmid; Monoclonal antibody; Immunoelectron microscopy; Quaternary structure; Tol plasmid; Protein-a; Pseudomonas; Immunoglobulins; Purification; Oxygenases; Microscopy; Metabolism; Binding; Pathway;

机译：儿茶酚2;3-双加氧酶;恶臭假单胞菌;Tol质粒;单克隆抗体;免疫电子显微镜;四级结构;Tol质粒;蛋白-a;假单胞菌;免疫球蛋白;纯化;氧合酶;显微术;代谢;结合;途径;

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1. TETRAMERIC STRUCTURE AND CELLULAR LOCATION OF CATECHOL 2,3-DIOXYGENASE [J] . Winkler J., Dwyer DF., Rohde M., Archives of microbiology . 1995,第1期

机译：儿茶酚2,3-二氧合酶的四聚体结构和细胞定位
2. Enzymatic activity of catechol 1,2-dioxygenase and catechol 2,3-dioxygenase produced by Gordonia polyisoprenivorans [J] . Andreazza Robson, Baldoni Daiana Bortoluzzi, Bento Fátima M., Quimica nova . 2012,第8期

机译：Gordonia polyisoprenivorans生产的邻苯二酚1,2-二加氧酶和邻苯二酚2,3-二加氧酶的酶活性
3. Enzymatic activity of catechol 1,2-dioxygenase and catechol 2,3-dioxygenase produced by Gordonia polyisoprenivorans [J] . Andreazza Robson, Baldoni Daiana Bortoluzzi, Bento Fátima M., Quimica nova . 2012,第8期

机译：Gordonia polyisoprenivorans生产的邻苯二酚1,2-二加氧酶和邻苯二酚2,3-二加氧酶的酶活性
4. Expression,Purification,and Characterization of a Catechol 2,3 dioxygenase from Bacillus ligniniphilus(PPT) [C] . Trent Yang PaperWeek Canada;BIOFOR International . 2020

机译：来自芽孢杆菌（PPT）的儿茶酚2,3 DiOxygenase的表达，纯化和表征
5. Catechol 2,3-dioxygenase-assisted cleavage of aromatics by "anaerobic" termite gut spirochetes and genomic evidence of a complete meta-pathway. [D] . Lucey, Kaitlyn Shae. 2014

机译：邻苯二酚2,3-二加氧酶通过“厌氧”白蚁肠道螺旋体裂解芳香族化合物和完整的元通路的基因组证据。
6. Discovery of Novel Inhibitors of Indoleamine 2,3-Dioxygenase 1 Through Structure-Based Virtual Screening [O] . Guoqing Zhang, Jing Xing, Yulan Wang, 2018

机译：通过基于结构的虚拟筛选发现吲哚胺2,3-双加氧酶1的新型抑制剂。
7. An archetypical extradiol-cleaving catecholic dioxygenase: the crystal structure of catechol 2,3-dioxygenase (metapyrocatechase) from Pseudomonas putida mt-2 [O] . Kita Akiko, Kita Shin-ichi, Fujisawa Ikuhide, 1999

机译：原型外二醇裂解的儿茶酚双加氧酶：恶臭假单胞菌mt-2的儿茶酚2，3-二加氧酶（间苯二酚酯酶）的晶体结构
8. DNA (Deoxyribonucleic Acids) Sequence of the Acinetobacter calcoaceticus Catechol 1,2-Dioxygenase I Structural Gene catA: Evidence for Evolutionary Divergence of Intradiol Dioxygenases by Acquisition of DNA Sequence Repetitions [R] . Neidle, E. L., Hartnett, C., Bonitz, S., 1988

机译：DNa（脱氧核糖核酸）醋酸钙不动杆菌儿茶酚1,2-双加氧酶I结构基因cata序列：通过获取DNa序列重复进化内二醇双加氧酶的进化分歧的证据

TETRAMERIC STRUCTURE AND CELLULAR LOCATION OF CATECHOL 2,3-DIOXYGENASE

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