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首页> 外文期刊>Archives of microbiology >CRYPTIC DEHALOGENASE AND CHLOROAMIDASE GENES IN PSEUDOMONAS PUTIDA AND THE INFLUENCE OF ENVIRONMENTAL CONDITIONS ON THEIR EXPRESSION
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CRYPTIC DEHALOGENASE AND CHLOROAMIDASE GENES IN PSEUDOMONAS PUTIDA AND THE INFLUENCE OF ENVIRONMENTAL CONDITIONS ON THEIR EXPRESSION

机译：假单胞菌中的密码酶和氯酰胺酶基因及其环境条件对其表达的影响

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摘要

Mutants of two strains of Pseudomonas putida expressed two cryptic chloroamidases (C-amidase and H-amidase) and one cryptic dehalogenase (DehII). The mutants were selected on either 2-chloropropionamide (2CPA) or 2-monochloropropionate (2MCPA), developing as papillae in parental colonies growing on a metabolisable support substrate. Mutants expressing C-amidase were selected if 2CPA was utilised as either a carbon or a nitrogen source. H-amidase mutants were selected only if 2CPA was used as a nitrogen source. Growth temperature and pH affected the frequency of papillae production, al though different temperatures and pHs did not affect the overall growth characteristics of the parental colonies. Decreasing growth temperature increased the frequency of 2cpa(+) papillae formation, but decreased the frequency of 2mcpa(+) papillae formation. Low pH (6.0) prevented the formation of 2mcpa(+) and 2cpa(+) papillae. However, in the case of the 2cpa(+) papillae, decreasing the growth temperature also allowed papillae formation at pH 6.0. [References: 30]
机译：两株恶臭假单胞菌的突变体表达了两种隐性氯酰胺酶（C-酰胺酶和H-酰胺酶）和一种隐性脱卤素酶（DehII）。在2-氯丙酰胺（2CPA）或2-一氯丙酸酯（2MCPA）上选择突变体，在可代谢支持底物上生长的亲本菌落中发育为乳头状。如果将2CPA用作碳源或氮源，则选择表达C-酰胺酶的突变体。仅当使用2CPA作为氮源时，才选择H-酰胺酶突变体。生长温度和pH值影响乳头产生的频率，尽管不同的温度和pH值不会影响亲代菌落的总体生长特征。生长温度降低会增加2cpa（+）乳头形成的频率，但会降低2mcpa（+）乳头形成的频率。低pH（6.0）阻止了2mcpa（+）和2cpa（+）乳头的形成。但是，在2cpa（+）乳头的情况下，降低生长温度也会在pH 6.0时形成乳头。 [参考：30]

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