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首页> 外文期刊>Archives of microbiology >Early candidacy for differentiation into heterocysts in the filamentous cyanobacterium Anabaena sp. PCC 7120.
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Early candidacy for differentiation into heterocysts in the filamentous cyanobacterium Anabaena sp. PCC 7120.

机译：早期候选资格分化为丝状蓝藻鱼腥藻中的杂种。 PCC 7120。

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The filamentous cyanobacterium Anabaena sp. PCC 7120 fixes dinitrogen facultatively. Upon depletion of combined nitrogen, about 10% of vegetative cells within the filaments differentiate terminally into nitrogen-fixing cells. The heterocyst has been studied as a model system of prokaryotic cell differentiation, with major focus on signal transduction and pattern formation. The fate of heterocyst differentiation is determined at about the eighth hour of induction (point of no return), well before conspicuous morphological or metabolic changes occur. However, little is known about how the initial heterocysts are selected after the induction by nitrogen deprivation. To address this question, we followed the fate of every cells on agar plates after nitrogen deprivation with an interval of 4 h. About 10% of heterocysts were formed without prior division after the start of nitrogen deprivation. The intensity of fluorescence of GFP in the transformants of hetR-gfp increased markedly in the future heterocysts at the fourth hour with respect to other cells. We also noted that the growing filaments consisted of clusters of four consecutive cells that we call quartets. About 75% of initial heterocysts originated from either of the two outer cells of quartets at the start of nitrogen deprivation. These results suggest that the future heterocysts are loosely selected at early times after the start of nitrogen deprivation, before the commitment. Such early candidacy could be explained by different properties of the outer and inner cells of a quartet, but the molecular nature of candidacy remains to be uncovered.
机译：丝状蓝细菌鱼腥藻。 PCC 7120可轻松固定二氮。消耗完的氮后，细丝内约10％的营养细胞最终分化为固氮细胞。已经研究了异胚囊作为原核细胞分化的模型系统，主要致力于信号转导和模式形成。异囊分化的命运在诱导的第8小时（不返回点）确定，很早就发生了明显的形态或代谢变化。但是，对于氮剥夺诱导后如何选择初始杂种囊，知之甚少。为了解决这个问题，我们追踪了氮剥夺后间隔4小时的琼脂平板上每个细胞的命运。氮剥夺开始后，没有事先分裂就形成了约10％的异质囊。相对于其他细胞，hetR-gfp转化子中GFP的荧光强度在未来的第四个小时的异胚囊中显着增加。我们还注意到，生长中的细丝由四个连续细胞的簇组成，我们称之为四重奏。在氮剥夺开始时，大约75％的初始异型囊来自四重奏的两个外部细胞中的任何一个。这些结果表明，在氮缺乏开始后的早期，在承诺之前，较松散地选择了未来的杂种。这样的早期候选资格可以通过四方组的外部和内部单元的不同属性来解释，但是候选资格的分子性质仍有待发现。

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