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首页> 外文期刊>Archives of microbiology >Genetic diversity of Rhizobium from nodulating beans grown in a variety of Mediterranean climate soils of Chile
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Genetic diversity of Rhizobium from nodulating beans grown in a variety of Mediterranean climate soils of Chile

机译：智利多种地中海气候土壤中生长的根瘤豆根瘤菌的遗传多样性

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In spite of potentially being an important source of rhizobial diversity and a key determinant of common bean productivity, there is a paucity of data on Rhizobium genetic variation and species composition in the important bean producing area of Chile and only one species has been documented (Rhizobium leguminosarum). In this study, 240 Rhizobium isolates from Torcaza bean (Phaseolus vulgaris L.) nodules established in the highest bean producing area in Chile (33A degrees 34'S-70A degrees 38'W and 37A degrees 36'S-71A degrees 47'W) were characterized by PCR-RFLP markers for nodC gene, revealing eight banding patterns with the polymorphic enzyme Hinf I. The locality of San Agustin de Aurora in Central Chile (35A degrees 32'S-71A degrees 29'W) had the highest level of diversity. Isolates were classified by species using PCR-RFLP markers for 16S rDNA gene and were confirmed by sequencing an internal fragment of the 16S rDNA gene. The results confirmed the presence of R. leguminosarum and three other species of rhizobia nodulating beans in South Central Chile (R. etli, R. tropici and R. leucaenae). R. tropici and R. leucaenae showed the least genetic variation and were most commonly identified in acid soils, while R. etli was the most common species in slightly acidic to moderately alkaline soils, with higher levels of organic matter content. R. leguminosarum was identified in almost all soils, was the most genetically diverse, and was the most common, being documented in soils with pH that ranged between 5.3 and 8.2, and with organic matter content between 2.1 and 4 %.
机译：尽管可能是根瘤菌多样性的重要来源和决定普通豆产量的关键因素，但智利重要豆类产区的根瘤菌遗传变异和物种组成方面的数据很少，而且仅记录了一个物种（根瘤菌豆科）。在这项研究中，在智利最高产豆区（33A度34'S-70A度38'W和37A度36'S-71A度47'W）建立的240个来自托卡萨豆（菜豆）结节的根瘤菌分离物的特征在于nodC基因的PCR-RFLP标记，揭示了多态性酶Hinf I的八种条带化模式。智利中部圣奥古斯丁·德奥罗拉的位置（35A度32'S-71A度29'W）具有最高的多样性水平。使用16S rDNA基因的PCR-RFLP标记按物种对分离物进行分类，并通过对16S rDNA基因的内部片段进行测序来确认。结果证实在智利中南部存在豆科根瘤菌和其他三种根瘤菌根瘤豆（R. etli，R。tropici和R. leucaenae）。 R. tropici和R. leucaenae遗传变异最少，在酸性土壤中最常见，而R. etli是在弱酸性至中度碱性土壤中最常见的物种，有机质含量较高。在几乎所有的土壤中都鉴定出豆科菌，在遗传上最多样化，也是最常见的，在pH值介于5.3和8.2之间且有机物含量在2.1和4％之间的土壤中有记载。

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