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首页> 外文期刊>Archives of microbiology >Reliable transformation system for Microbotryum lychnidis-dioicae informed by genome and transcriptome project
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Reliable transformation system for Microbotryum lychnidis-dioicae informed by genome and transcriptome project

机译：基因组和转录组项目指导的可靠的荔枝双歧杆菌转化系统

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摘要

Microbotryum lychnidis-dioicae is a member of a species complex infecting host plants in the Caryophyllaceae. It is used as a model system in many areas of research, but attempts to make this organism tractable for reverse genetic approaches have not been fruitful. Here, we exploited the recently obtained genome sequence and transcriptome analysis to inform our design of constructs for use in Agrobacterium-mediated transformation techniques currently available for other fungi. Reproducible transformation was demonstrated at the genomic, transcriptional and functional levels. Moreover, these initial proof-of-principle experiments provide evidence that supports the findings from initial global transcriptome analysis regarding expression from the respective promoters under different growth conditions of the fungus. The technique thus provides for the first time the ability to stably introduce transgenes and over-express target M. lychnidis-dioicae genes.
机译：lychnidis-dioicae Microbotryum lychnidis-dioicae是感染石竹科的宿主植物的物种复合体的成员。它被用作许多研究领域的模型系统，但是试图使这种生物易于逆向遗传方法的尝试并未取得成果。在这里，我们利用了最近获得的基因组序列和转录组分析，以指导我们设计用于目前可用于其他真菌的农杆菌介导的转化技术的构建体。在基因组，转录和功能水平上证明了可再现的转化。而且，这些最初的原理证明实验提供了证据，支持有关在不同的真菌生长条件下来自各个启动子的表达的初始全局转录组分析的发现。因此，该技术首次提供了稳定引入转基因和过表达靶点M. lychnidis-dioicae基因的能力。

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