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首页> 外文期刊>Archives of microbiology >Functional characterization of glucosamine-6-phosphate synthase (GlmS) in Salmonella enterica serovar Enteritidis
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Functional characterization of glucosamine-6-phosphate synthase (GlmS) in Salmonella enterica serovar Enteritidis

机译：肠炎沙门氏菌肠炎沙门氏菌中氨基葡萄糖-6-磷酸合酶（GlmS）的功能表征

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摘要

Salmonella is a threat to public health due to consumption of contaminated food. Screening of a transposon library identified a unique mutant that was growth and host cell binding deficient. The objective of this study was to determine the functional role of glucosamine-6-phosphate synthase (GlmS) in the biology and pathogenesis of Salmonella. To examine this, we created a glmS mutant (Delta glmS) of Salmonella and examined the effect on cell envelope integrity, growth, metabolism, and pathogenesis. Our data indicated Delta glmS was defective in growth unless media were supplemented with d-glucosamine (d-GlcN). Examination of the bacterial cell envelope revealed that Delta glmS was highly sensitive to detergents, hydrophobic antibiotics, and bile salts compared to the wild type (WT). A release assay indicated that Delta glmS secreted higher amounts of beta-lactamase than the WT in culture supernatant fractions. Furthermore, Delta glmS was attenuated in cell culture models of Salmonella infection. Taken together, this study determined an important role for GlmS in the pathogenesis and biology of Salmonella.
机译：沙门氏菌由于食用受污染的食物而对公共健康构成威胁。转座子文库的筛选确定了独特的突变体，其生长和宿主细胞结合缺陷。这项研究的目的是确定氨基葡萄糖6磷酸合酶（GlmS）在沙门氏菌的生物学和发病机理中的功能作用。为了检查这一点，我们创建了沙门氏菌的glmS突变体（Delta glmS），并检查了对细胞膜完整性，生长，代谢和发病机理的影响。我们的数据表明，除非培养基中添加了d-葡萄糖胺（d-GlcN），否则Delta glmS在生长上是有缺陷的。细菌细胞包膜的检查显示，与野生型（WT）相比，Delta glmS对去污剂，疏水性抗生素和胆汁盐高度敏感。释放测定表明，在培养上清液级分中，Delta glmS分泌的β-内酰胺酶量比WT高。此外，在沙门氏菌感染的细胞培养模型中，Delta glmS减弱了。综上所述，这项研究确定了GlmS在沙门氏菌的发病机理和生物学中的重要作用。

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