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Expression of the succinate dehydrogenase genes (sdhCAB) from the facultatively anaerobic Paenibacillus macerans during aerobic growth

机译:兼性厌氧性Macerans有氧生长过程中琥珀酸脱氢酶基因(sdhCAB)的表达

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Paenibacillus (formerly Bacillus) macerans is capable of succinate oxidation under oxic conditions and fumarate reduction under anoxic conditions. The reactions are catalyzed by different enzymes, succinate dehydrogenase (Sdh) and fumarate reductase (Frd). The genes encoding Sdh (sdhCAB) were analyzed. The gene products of sdhA and sdhB were similar to the subunits of known Sdh and Frd enzymes. The hydrophobic subunit SdhC showed close sequence similarity to the class of Sdh/Frd enzymes containing diheme cytochrome b. From the sdhCAB gene cluster two transcripts were produced, one comprising sdhCAB, the other sdhAB. The transcripts were found only during aerobic growth, and the amount was directly proportional to Sdh activity, but inversely proportional to Frd activity. [References: 24]
机译:潘氏芽孢杆菌(以前的芽孢杆菌)能够在有氧条件下琥珀酸氧化并在无氧条件下还原富马酸酯。该反应由不同的酶,琥珀酸脱氢酶(Sdh)和富马酸还原酶(Frd)催化。分析了编码Sdh(sdhCAB)的基因。 sdhA和sdhB的基因产物类似于已知Sdh和Frd酶的亚基。疏水性亚基SdhC与包含双血红素细胞色素b的Sdh / Frd酶类别具有相似的序列相似性。从sdhCAB基因簇中产生了两个转录本,一个包含sdhCAB,另一个包含sdhAB。仅在有氧生长期间才发现转录本,其数量与Sdh活性成正比,而与Frd活性成反比。 [参考:24]

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