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首页> 外文期刊>Archives of Oral Biology >FGF-2 induces proliferation of human periodontal ligament cells and maintains differentiation potentials of STRO-1 +/CD146 + periodontal ligament cells
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FGF-2 induces proliferation of human periodontal ligament cells and maintains differentiation potentials of STRO-1 +/CD146 + periodontal ligament cells

机译：FGF-2诱导人牙周膜细胞增殖并维持STRO-1 + / CD146 +牙周膜细胞的分化潜能

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The presence of human STRO-1 +/CD146 + periodontal ligament (PDL) cells has been reported, but obtaining a large amount of these cells is difficult. The purpose of this study was to evaluate the percentages of STRO-1 +/CD146 + cells in PDL cells and determine the effects of FGF-2 on the proliferation and multilineage differentiation potency of these cells. Human PDL (HPDL) cells were individually prepared from 15 extracted teeth. HPDL cells were cultured with or without FGF-2, and the percentages of STRO-1 +/CD146 + cells in each HPDL cell culture was examined using FACSAria?. The STRO-1 +/CD146 + cells were sorted with FACSAria?, and the mRNA expression and differentiation potency of the sorted cells were subsequently examined. The numbers of the STRO-1 +/CD146 + cells in the FGF-2 cultures were significantly higher than those cultured in the absence of FGF-2. The sorted STRO-1 +/CD146 + cells expressed mRNA of PDL markers and differentiated into adipocytes and osteoblast-like cells. The present study shows that FGF-2 augmented the proliferation of the STRO-1 +/ CD146 + cells in the HPDL cultures whilst retaining adipogenic and osteogenic differentiation potentials. Thus, it may be useful to culture HPDL cells with FGF-2 for the application of the human STRO-1 +/CD146 + PDL cells in periodontal tissue regeneration.
机译：已经报道了人STRO-1 + / CD146 +牙周膜（PDL）细胞的存在，但是很难获得大量这些细胞。这项研究的目的是评估PDL细胞中STRO-1 + / CD146 +细胞的百分比，并确定FGF-2对这些细胞的增殖和多系分化潜能的影响。从15个拔出的牙齿中分别制备人PDL（HPDL）细胞。在有或没有FGF-2的情况下培养HPDL细胞，并使用FACSAria？检测每种HPDL细胞培养物中STRO-1 + / CD146 +细胞的百分比。用FACSAria？分选STRO-1 + / CD146 +细胞，随后检查分选细胞的mRNA表达和分化潜能。 FGF-2培养物中STRO-1 + / CD146 +细胞的数量明显高于不存在FGF-2时培养的数量。分选的STRO-1 + / CD146 +细胞表达PDL标记的mRNA，并分化为脂肪细胞和成骨样细胞。本研究表明，FGF-2增强了HPDL培养物中STRO-1 + / CD146 +细胞的增殖，同时保留了成脂和成骨分化潜能。因此，用FGF-2培养HPDL细胞对于将人STRO-1 + / CD146 + PDL细胞应用于牙周组织再生中可能是有用的。

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